Three methods for the detection of endosymbionts were compared on Diuraphis noxia (Aphididae) and 8 genera of Adelgidae. Out of these methods (diagnostic PCR, RFLP, DGGE), DGGE was the most successful. In populations of Diuraphis noxia 12 bacterial species were detected with no geografical pattern but relation to host plant was suggested. In adelgids 11 species of bacteria were detected with no obvious pattern of coevolution.
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Length of the covering note
59 s.
Language
CZ
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Annotation in English
Three methods for the detection of endosymbionts were compared on Diuraphis noxia (Aphididae) and 8 genera of Adelgidae. Out of these methods (diagnostic PCR, RFLP, DGGE), DGGE was the most successful. In populations of Diuraphis noxia 12 bacterial species were detected with no geografical pattern but relation to host plant was suggested. In adelgids 11 species of bacteria were detected with no obvious pattern of coevolution.