This master thesis aimed to develop a system to sort individual life cycle stages of the mammalian parasite Trypanosoma brucei generated in vitro. T. brucei cell lines expressing developmentally regulatable fluorescent protein due to the stage specific 3'UTRs were generated. Furthermore, a double knock-out of a protein (TbIF1) presumably involved in the parasite's differentiation was attempted.
Anotace v angličtině
This master thesis aimed to develop a system to sort individual life cycle stages of the mammalian parasite Trypanosoma brucei generated in vitro. T. brucei cell lines expressing developmentally regulatable fluorescent protein due to the stage specific 3'UTRs were generated. Furthermore, a double knock-out of a protein (TbIF1) presumably involved in the parasite's differentiation was attempted.
Klíčová slova
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Klíčová slova v angličtině
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Rozsah průvodní práce
53 s.
Jazyk
AN
Anotace
This master thesis aimed to develop a system to sort individual life cycle stages of the mammalian parasite Trypanosoma brucei generated in vitro. T. brucei cell lines expressing developmentally regulatable fluorescent protein due to the stage specific 3'UTRs were generated. Furthermore, a double knock-out of a protein (TbIF1) presumably involved in the parasite's differentiation was attempted.
Anotace v angličtině
This master thesis aimed to develop a system to sort individual life cycle stages of the mammalian parasite Trypanosoma brucei generated in vitro. T. brucei cell lines expressing developmentally regulatable fluorescent protein due to the stage specific 3'UTRs were generated. Furthermore, a double knock-out of a protein (TbIF1) presumably involved in the parasite's differentiation was attempted.