Four negative stains, hafnium chloride and europium, samarium and gadolinium nitrates, were tested in single particle electron microscopy as potential alternatives to uranyl acetate, which is recently being widely restricted for its toxicity. The new stains were applied to a structurally well-described plant photosystem I, visualized by a transmission electron microscope and classified in a single particle analysis. The quality of the stains was evaluated by the obtained resolution and ability to provide reliable structural information.
Anotace v angličtině
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Klíčová slova
Uranyl acetate, lanthanides, negative staining, single particle analysis, electron microscopy
Klíčová slova v angličtině
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Rozsah průvodní práce
50 s. (89 170 znaků vč. mezer)
Jazyk
AN
Anotace
Four negative stains, hafnium chloride and europium, samarium and gadolinium nitrates, were tested in single particle electron microscopy as potential alternatives to uranyl acetate, which is recently being widely restricted for its toxicity. The new stains were applied to a structurally well-described plant photosystem I, visualized by a transmission electron microscope and classified in a single particle analysis. The quality of the stains was evaluated by the obtained resolution and ability to provide reliable structural information.
Anotace v angličtině
-
Klíčová slova
Uranyl acetate, lanthanides, negative staining, single particle analysis, electron microscopy