The aim of the study is a comparison of five distinct pipelines for environmental DNA (eDNA) metabarcoding using data collected in three reservoirs (Klíčava, Římov, and Žlutice) in the summer and autumn seasons. The results are analysed by comparing the number of reads assigned, number of species detected, and ecological indices (alpha and beta diversity). Finally, statistical analysis is applied to corroborate the results using analysis of variance (ANOVA), post-hoc Tukey, and permutational multivariate analysis of variance (PERMANOVA).
Anotace v angličtině
The aim of the study is a comparison of five distinct pipelines for environmental DNA (eDNA) metabarcoding using data collected in three reservoirs (Klíčava, Římov, and Žlutice) in the summer and autumn seasons. The results are analysed by comparing the number of reads assigned, number of species detected, and ecological indices (alpha and beta diversity). Finally, statistical analysis is applied to corroborate the results using analysis of variance (ANOVA), post-hoc Tukey, and permutational multivariate analysis of variance (PERMANOVA).
Klíčová slova
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Klíčová slova v angličtině
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Rozsah průvodní práce
201 p.
Jazyk
AN
Anotace
The aim of the study is a comparison of five distinct pipelines for environmental DNA (eDNA) metabarcoding using data collected in three reservoirs (Klíčava, Římov, and Žlutice) in the summer and autumn seasons. The results are analysed by comparing the number of reads assigned, number of species detected, and ecological indices (alpha and beta diversity). Finally, statistical analysis is applied to corroborate the results using analysis of variance (ANOVA), post-hoc Tukey, and permutational multivariate analysis of variance (PERMANOVA).
Anotace v angličtině
The aim of the study is a comparison of five distinct pipelines for environmental DNA (eDNA) metabarcoding using data collected in three reservoirs (Klíčava, Římov, and Žlutice) in the summer and autumn seasons. The results are analysed by comparing the number of reads assigned, number of species detected, and ecological indices (alpha and beta diversity). Finally, statistical analysis is applied to corroborate the results using analysis of variance (ANOVA), post-hoc Tukey, and permutational multivariate analysis of variance (PERMANOVA).
Klíčová slova
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Klíčová slova v angličtině
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Zásady pro vypracování
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Zásady pro vypracování
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Seznam doporučené literatury
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Seznam doporučené literatury
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Přílohy volně vložené
Tables, Codes, reference database in genbank format, and configuration files.