Informace o kvalifikační práci Validation of mitochondrial localization and essentiality of prioritized proteins assigned to the tripartite attachment complex in the protozoan parasite Trypanosoma brucei
- Pro tuto VŠKP nejsou definovány žádné údaje, u kterých by bylo požadováno jejich vyplnění.
Hlavní téma
Validation of mitochondrial localization and essentiality of prioritized proteins assigned to the tripartite attachment complex in the protozoan parasite Trypanosoma brucei
Hlavní téma v angličtině
Validation of mitochondrial localization and essentiality of prioritized proteins assigned to the tripartite attachment complex in the protozoan parasite Trypanosoma brucei
Název dle studenta
Validation of mitochondrial localization and essentiality of prioritized proteins assigned to the tripartite attachment complex in the protozoan parasite Trypanosoma brucei
Název dle studenta v angličtině
Validation of mitochondrial localization and essentiality of prioritized proteins assigned to the tripartite attachment complex in the protozoan parasite Trypanosoma brucei
During this thesis the localization of the prioritized proteins Tb927.11.13600, Tb927.11.14570, Tb927.4.840 and Tb927.6.4540 and their connection to the tripartite attachment complex of the protozoan parasite Trypanosoma brucei was examined to verify previous annotations found on the TrypTag database. Additionally, the essentiality of the prioritized proteins was evaluated.
Anotace v angličtině
During this thesis the localization of the prioritized proteins Tb927.11.13600, Tb927.11.14570, Tb927.4.840 and Tb927.6.4540 and their connection to the tripartite attachment complex of the protozoan parasite Trypanosoma brucei was examined to verify previous annotations found on the TrypTag database. Additionally, the essentiality of the prioritized proteins was evaluated.
Klíčová slova
Trypanosoma brucei, tripartite attachment complex, mitochondrion, putative protein, Tryptag, parasite, protein essentiality, protein localization, endogenous tagging, RNA interference
Klíčová slova v angličtině
Trypanosoma brucei, tripartite attachment complex, mitochondrion, putative protein, Tryptag, parasite, protein essentiality, protein localization, endogenous tagging, RNA interference
Rozsah průvodní práce
51 (82 852 characters)
Jazyk
AN
Anotace
During this thesis the localization of the prioritized proteins Tb927.11.13600, Tb927.11.14570, Tb927.4.840 and Tb927.6.4540 and their connection to the tripartite attachment complex of the protozoan parasite Trypanosoma brucei was examined to verify previous annotations found on the TrypTag database. Additionally, the essentiality of the prioritized proteins was evaluated.
Anotace v angličtině
During this thesis the localization of the prioritized proteins Tb927.11.13600, Tb927.11.14570, Tb927.4.840 and Tb927.6.4540 and their connection to the tripartite attachment complex of the protozoan parasite Trypanosoma brucei was examined to verify previous annotations found on the TrypTag database. Additionally, the essentiality of the prioritized proteins was evaluated.
Klíčová slova
Trypanosoma brucei, tripartite attachment complex, mitochondrion, putative protein, Tryptag, parasite, protein essentiality, protein localization, endogenous tagging, RNA interference
Klíčová slova v angličtině
Trypanosoma brucei, tripartite attachment complex, mitochondrion, putative protein, Tryptag, parasite, protein essentiality, protein localization, endogenous tagging, RNA interference
Zásady pro vypracování
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Zásady pro vypracování
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Seznam doporučené literatury
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Seznam doporučené literatury
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Přílohy volně vložené
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Přílohy vázané v práci
ilustrace, grafy, schémata, tabulky
Převzato z knihovny
Ne
Plný text práce
Přílohy
Posudek(y) oponenta
Hodnocení vedoucího
Záznam průběhu obhajoby
The student, commission members, and the audience were welcomed by the head of the commission, prof. Grubhoffer. The student described the studied organism and the studied complex, the tripartite attachment complex and its components. Next, the aims and the experimental approach were described, followed by the results, which were thoroughly discussed.
The supervisor, Dr Durante presented his review followed by the opponent, Dr Benz. The student answered all of the opponent's questions to her satisfaction. Next, the student answered questions from the commission on the chosen proteins and checking the effectivity of RNAi by qRT-PCR.
5x excellent - final excellent.
The commission suggests this work for an Award by the head of the Dept. of Chemistry.