The objective of this thesis is to investigate the efficiency of the antimicrobial peptide anisaxin-1, from the zoonotic nematodes of the Anisakis genus, towards Borrelia burgdorferi sensu stricto at different concentrations. We aimed to compare its antimicrobial properties with other anti-borrelial agents, including melittin, an antimicrobial agent found in bee venom, and the conventional antibiotics doxycycline, cefoperazone, and daptomycin. It is important to identify a new natural compound that might be used for Lyme disease treatment due to the bacterial ability to develop resistance to the antibiotics. Antimicrobial peptides have been recognised as compounds that do not trigger persistent antimicrobial resistance. The analysis was performed using the live/dead bacterial staining method, fluorescent microscopy, statistical testing, and comparison with previously published data. To test statistical significance among treatments, ANOVA and Tukey-HSD were employed to determine the effect of the anisaxin at different concentrations.
Anotace v angličtině
The objective of this thesis is to investigate the efficiency of the antimicrobial peptide anisaxin-1, from the zoonotic nematodes of the Anisakis genus, towards Borrelia burgdorferi sensu stricto at different concentrations. We aimed to compare its antimicrobial properties with other anti-borrelial agents, including melittin, an antimicrobial agent found in bee venom, and the conventional antibiotics doxycycline, cefoperazone, and daptomycin. It is important to identify a new natural compound that might be used for Lyme disease treatment due to the bacterial ability to develop resistance to the antibiotics. Antimicrobial peptides have been recognised as compounds that do not trigger persistent antimicrobial resistance. The analysis was performed using the live/dead bacterial staining method, fluorescent microscopy, statistical testing, and comparison with previously published data. To test statistical significance among treatments, ANOVA and Tukey-HSD were employed to determine the effect of the anisaxin at different concentrations.
The objective of this thesis is to investigate the efficiency of the antimicrobial peptide anisaxin-1, from the zoonotic nematodes of the Anisakis genus, towards Borrelia burgdorferi sensu stricto at different concentrations. We aimed to compare its antimicrobial properties with other anti-borrelial agents, including melittin, an antimicrobial agent found in bee venom, and the conventional antibiotics doxycycline, cefoperazone, and daptomycin. It is important to identify a new natural compound that might be used for Lyme disease treatment due to the bacterial ability to develop resistance to the antibiotics. Antimicrobial peptides have been recognised as compounds that do not trigger persistent antimicrobial resistance. The analysis was performed using the live/dead bacterial staining method, fluorescent microscopy, statistical testing, and comparison with previously published data. To test statistical significance among treatments, ANOVA and Tukey-HSD were employed to determine the effect of the anisaxin at different concentrations.
Anotace v angličtině
The objective of this thesis is to investigate the efficiency of the antimicrobial peptide anisaxin-1, from the zoonotic nematodes of the Anisakis genus, towards Borrelia burgdorferi sensu stricto at different concentrations. We aimed to compare its antimicrobial properties with other anti-borrelial agents, including melittin, an antimicrobial agent found in bee venom, and the conventional antibiotics doxycycline, cefoperazone, and daptomycin. It is important to identify a new natural compound that might be used for Lyme disease treatment due to the bacterial ability to develop resistance to the antibiotics. Antimicrobial peptides have been recognised as compounds that do not trigger persistent antimicrobial resistance. The analysis was performed using the live/dead bacterial staining method, fluorescent microscopy, statistical testing, and comparison with previously published data. To test statistical significance among treatments, ANOVA and Tukey-HSD were employed to determine the effect of the anisaxin at different concentrations.
The head of the commission Prof Vácha welcomed the student Ms Kurbegović, members of the committee, and guests. All committee members were present.
The student presented the theoretical background on Lyme disease borrelia, its spread in Europe and the Earth, antimicrobial peptides, Anisakis pegrefii, anisaxins, the project aims, the experimental setup, methods used, and results.
The supervisor and the opponent presented their reviews and the student answered the questions and comments and further discussed various topics with the opponent.
Discussion with the commission members followed on the mode of action of various antimicrobial peptides, the benefits of antimicrobial peptides over antibiotics and the practical use in terms of proteolytic degradation, cytotoxicity experiments on the used antimicrobial peptides, the possible bacteriostatic effect of the tested antimicrobial peptides, the experimental setup and the average results, the statistical analyses, resistance in borrelia. The student answered all questions.
6x excellent, final - excellent
The commission suggested this thesis to be awarded by the head of the Department.