Sphaerospora molnari is a myxozoan parasite causing systemic immune responses and hemolytic anemia in Cyprinus carpio. This study aimed to stimulate protective immunity against S. molnari through the administration of a probiotic treatment containing -GAL-rich Escherichia coli strain (Nissle 1917). Feed enriched with varying doses of lyophilized bacteria was used alongside gavage and intraperitoneal injection methods in six treatment groups. Infection progression was monitored through flow cytometry, qPCR assays, and ELISA tests for antibody titers. Results showed that the E. coli vaccine provided immediate protective immunity, reducing the severity of hemolytic anemia. The findings of this study provide valuable knowledge for future research and development of a probiotic in-feed product against parasitic infections of fish.
Anotace v angličtině
Sphaerospora molnari is a myxozoan parasite causing systemic immune responses and hemolytic anemia in Cyprinus carpio. This study aimed to stimulate protective immunity against S. molnari through the administration of a probiotic treatment containing -GAL-rich Escherichia coli strain (Nissle 1917). Feed enriched with varying doses of lyophilized bacteria was used alongside gavage and intraperitoneal injection methods in six treatment groups. Infection progression was monitored through flow cytometry, qPCR assays, and ELISA tests for antibody titers. Results showed that the E. coli vaccine provided immediate protective immunity, reducing the severity of hemolytic anemia. The findings of this study provide valuable knowledge for future research and development of a probiotic in-feed product against parasitic infections of fish.
Sphaerospora molnari is a myxozoan parasite causing systemic immune responses and hemolytic anemia in Cyprinus carpio. This study aimed to stimulate protective immunity against S. molnari through the administration of a probiotic treatment containing -GAL-rich Escherichia coli strain (Nissle 1917). Feed enriched with varying doses of lyophilized bacteria was used alongside gavage and intraperitoneal injection methods in six treatment groups. Infection progression was monitored through flow cytometry, qPCR assays, and ELISA tests for antibody titers. Results showed that the E. coli vaccine provided immediate protective immunity, reducing the severity of hemolytic anemia. The findings of this study provide valuable knowledge for future research and development of a probiotic in-feed product against parasitic infections of fish.
Anotace v angličtině
Sphaerospora molnari is a myxozoan parasite causing systemic immune responses and hemolytic anemia in Cyprinus carpio. This study aimed to stimulate protective immunity against S. molnari through the administration of a probiotic treatment containing -GAL-rich Escherichia coli strain (Nissle 1917). Feed enriched with varying doses of lyophilized bacteria was used alongside gavage and intraperitoneal injection methods in six treatment groups. Infection progression was monitored through flow cytometry, qPCR assays, and ELISA tests for antibody titers. Results showed that the E. coli vaccine provided immediate protective immunity, reducing the severity of hemolytic anemia. The findings of this study provide valuable knowledge for future research and development of a probiotic in-feed product against parasitic infections of fish.
Dr Štěrba welcomed the student and commission members; opponent Dr Štěrbová and the supervisor Dr Holzer were not present; the faculty guarantor Dr Fiala was present during the defence. The student presented the theoretical background of his work on Myxozoa and fish infections, the aGal epitope and the process and hypothesis, followed by the experimental part and the results. Dr Fiala presented his review, and the opponent review was read by Dr Věchtová. The student answered all the questions of the opponent and also questions from the commission members on the doublets, possibilities of vaccination or in-feed vaccination, the determination of the titer and the process of data evaluation, and the detection of anti-aGal antibodies.
Votes: 3x 1, 1x 2
Final grade: 1
Points: 130