This bachelor thesis delves into the conservation and diversification of Polycomb Repressive Complex (PRC2) subunits in the model moss Physcomitrium patens. Through in-silico studies, gene expression analysis, and yeast two-hybrid experiments, it highlights the evolutionary relationships, gene expression patterns in distinct developmental phases, and interactions of PRC2 subunits. The findings extend our comprehension of the role that PRC2 plays in moss development and establish a platform for further exploration, extending our understanding of PRC2 engagement in plant development.
Anotace v angličtině
This bachelor thesis delves into the conservation and diversification of Polycomb Repressive Complex (PRC2) subunits in the model moss Physcomitrium patens. Through in-silico studies, gene expression analysis, and yeast two-hybrid experiments, it highlights the evolutionary relationships, gene expression patterns in distinct developmental phases, and interactions of PRC2 subunits. The findings extend our comprehension of the role that PRC2 plays in moss development and establish a platform for further exploration, extending our understanding of PRC2 engagement in plant development.
This bachelor thesis delves into the conservation and diversification of Polycomb Repressive Complex (PRC2) subunits in the model moss Physcomitrium patens. Through in-silico studies, gene expression analysis, and yeast two-hybrid experiments, it highlights the evolutionary relationships, gene expression patterns in distinct developmental phases, and interactions of PRC2 subunits. The findings extend our comprehension of the role that PRC2 plays in moss development and establish a platform for further exploration, extending our understanding of PRC2 engagement in plant development.
Anotace v angličtině
This bachelor thesis delves into the conservation and diversification of Polycomb Repressive Complex (PRC2) subunits in the model moss Physcomitrium patens. Through in-silico studies, gene expression analysis, and yeast two-hybrid experiments, it highlights the evolutionary relationships, gene expression patterns in distinct developmental phases, and interactions of PRC2 subunits. The findings extend our comprehension of the role that PRC2 plays in moss development and establish a platform for further exploration, extending our understanding of PRC2 engagement in plant development.
The head of the commission welcome the student and the members of the commission, all members of the commission except the supervisor and Dr Chmelař were present. The student presented the studied plant and the studied molecules, the aims, methods used and results on the identified genes, their characterization, and interactions, and discussed the results.
The supervisor and the opponent presented their reviews, and the student answered the opponent’s questions. Then, the student answered the questions from the commission on the use of a semi-quantitative PCR and the information it provides, the conservation between the genes and the use of primers, the possibilities to use overexpression and gene knockout in plants and comparison with Drosophila. The student answered only one of the questions satisfactorily, mostly the supervisor’s help was needed.
1x excellent, 3x very good, 1x good, final very good