Flow cytometry is an important method in various science fields, whereas in botany it is mostly used for genome size and ploidy estimation. In the last two decades research was conducted on the influence of different storage conditions on the quality of flow cytometry histograms and reliability of the genome size estimation for angiosperms. Thus, this thesis is focused on the influence of storage time and different types of storage by desiccation on the quality of the obtained flow cytometry histograms and genome size estimation reliability in selected
ferns and lycophytes.
Anotace v angličtině
Flow cytometry is an important method in various science fields, whereas in botany it is mostly used for genome size and ploidy estimation. In the last two decades research was conducted on the influence of different storage conditions on the quality of flow cytometry histograms and reliability of the genome size estimation for angiosperms. Thus, this thesis is focused on the influence of storage time and different types of storage by desiccation on the quality of the obtained flow cytometry histograms and genome size estimation reliability in selected
ferns and lycophytes.
Klíčová slova
flow cytometry, ferns and lycophytes, herbarium, silica gel, storage by desiccation, DAPI
Klíčová slova v angličtině
flow cytometry, ferns and lycophytes, herbarium, silica gel, storage by desiccation, DAPI
Rozsah průvodní práce
50 s (103 309 znaků)
Jazyk
AN
Anotace
Flow cytometry is an important method in various science fields, whereas in botany it is mostly used for genome size and ploidy estimation. In the last two decades research was conducted on the influence of different storage conditions on the quality of flow cytometry histograms and reliability of the genome size estimation for angiosperms. Thus, this thesis is focused on the influence of storage time and different types of storage by desiccation on the quality of the obtained flow cytometry histograms and genome size estimation reliability in selected
ferns and lycophytes.
Anotace v angličtině
Flow cytometry is an important method in various science fields, whereas in botany it is mostly used for genome size and ploidy estimation. In the last two decades research was conducted on the influence of different storage conditions on the quality of flow cytometry histograms and reliability of the genome size estimation for angiosperms. Thus, this thesis is focused on the influence of storage time and different types of storage by desiccation on the quality of the obtained flow cytometry histograms and genome size estimation reliability in selected
ferns and lycophytes.
Klíčová slova
flow cytometry, ferns and lycophytes, herbarium, silica gel, storage by desiccation, DAPI
Klíčová slova v angličtině
flow cytometry, ferns and lycophytes, herbarium, silica gel, storage by desiccation, DAPI
Zásady pro vypracování
-
Zásady pro vypracování
-
Seznam doporučené literatury
-
Seznam doporučené literatury
-
Přílohy volně vložené
1 Excel book
Přílohy vázané v práci
grafy, tabulky
Převzato z knihovny
Ne
Plný text práce
Přílohy
Posudek(y) oponenta
Hodnocení vedoucího
Záznam průběhu obhajoby
The head of the commission Prof Vácha welcomed the student Mr Šimeček, members of the committee, and guests. All committee members were present except David Kaftan and the opponent Dr. Lučanová.
The student presented the theoretical background on the sample types analysed in botany and the methods which could be used for genome analysis, various samples which were analysed, methods used, and results which were discussed.
The supervisor presented his review and the opponent's review was read by Dr Štěrba. The student sent his answers to the opponent in advance and she was satisfied with the answers.
Discussion with the commission members followed on the genome standard use, the reason, why the DNA is more degraded/the samples can be used for a longer time in some species, the possibilities to use flow cytometry, the reasons to determine the genome size in archived samples, correlation of non-measurable samples and DNA degradation, the presence of saponins in various species, the use of fixatives. The student answered all questions.
4x excellent, final - excellent
The commission recommends this thesis for the Award by the head of the Department