Informace o kvalifikační práci Differential expression of genes in replicative (spiral) and persistent (biofilms) forms of the causative agent of human Lyme borreliosis
The onset and development of post treatment Lyme disease syndrome (PTLDS) remains a constant challenge in the diagnosis and treatment of Lyme borreliosis (LB). The driving force behind this is the formation of persisters cells, which are able to hide within the host, "invisible" to the immune system and resistant to antibiotic treatment. Thus far, few types of persisters have been found to be formed by the Borrelia burgdorferi spirochete, with biofilms being one of them. Multiple atypical forms of spirochetes often are uneasy to be detected via the average testing methods, leading to many cases being misdiagnosed. Additionally, the formation of biofilms, as a resilient complex community of cells, has not only been observed in case of Lyme disease, but also in other illnesses worldwide. This thesis deals with the analysis of genes differentially expressed in biofilms in comparison to the replicating forms of spirochetes, with aim to better understand the formation of these morphotypes.
Anotace v angličtině
The onset and development of post treatment Lyme disease syndrome (PTLDS) remains a constant challenge in the diagnosis and treatment of Lyme borreliosis (LB). The driving force behind this is the formation of persisters cells, which are able to hide within the host, "invisible" to the immune system and resistant to antibiotic treatment. Thus far, few types of persisters have been found to be formed by the Borrelia burgdorferi spirochete, with biofilms being one of them. Multiple atypical forms of spirochetes often are uneasy to be detected via the average testing methods, leading to many cases being misdiagnosed. Additionally, the formation of biofilms, as a resilient complex community of cells, has not only been observed in case of Lyme disease, but also in other illnesses worldwide. This thesis deals with the analysis of genes differentially expressed in biofilms in comparison to the replicating forms of spirochetes, with aim to better understand the formation of these morphotypes.
The onset and development of post treatment Lyme disease syndrome (PTLDS) remains a constant challenge in the diagnosis and treatment of Lyme borreliosis (LB). The driving force behind this is the formation of persisters cells, which are able to hide within the host, "invisible" to the immune system and resistant to antibiotic treatment. Thus far, few types of persisters have been found to be formed by the Borrelia burgdorferi spirochete, with biofilms being one of them. Multiple atypical forms of spirochetes often are uneasy to be detected via the average testing methods, leading to many cases being misdiagnosed. Additionally, the formation of biofilms, as a resilient complex community of cells, has not only been observed in case of Lyme disease, but also in other illnesses worldwide. This thesis deals with the analysis of genes differentially expressed in biofilms in comparison to the replicating forms of spirochetes, with aim to better understand the formation of these morphotypes.
Anotace v angličtině
The onset and development of post treatment Lyme disease syndrome (PTLDS) remains a constant challenge in the diagnosis and treatment of Lyme borreliosis (LB). The driving force behind this is the formation of persisters cells, which are able to hide within the host, "invisible" to the immune system and resistant to antibiotic treatment. Thus far, few types of persisters have been found to be formed by the Borrelia burgdorferi spirochete, with biofilms being one of them. Multiple atypical forms of spirochetes often are uneasy to be detected via the average testing methods, leading to many cases being misdiagnosed. Additionally, the formation of biofilms, as a resilient complex community of cells, has not only been observed in case of Lyme disease, but also in other illnesses worldwide. This thesis deals with the analysis of genes differentially expressed in biofilms in comparison to the replicating forms of spirochetes, with aim to better understand the formation of these morphotypes.
Prof Grubhoffer welcomed the student and commission members; opponent Dr Kazimírová was absent. The student presented the theoretical background of her work on Lyme disease spirochetes, followed by the experimental part on the dormant forms and the detection of differentially expressed genes in dormant vs active forms of spirochetes and the results. The co-supervisor, Dr Rudenko, presented her review, and Dr Štěrba read the opponent's review. The student answered the opponent's question in the review and the comments and questions from the corrected thesis. Next, the student answered questions from the commission members on the role of tested proteins, counting the borrelia in biofilm, the presence of OspB in the identified proteins, the difference between the proteomics and transcriptomics results and the borrelia viability assession. The student answered all the questions.
Votes: 5x 1
Final grade: excellent
Points: 145
The commission suggests this thesis for the Outstanding bachelor theses award by the head of the Department of Chemistry.