TAL nucleases are frequently used for mutagenesis in germline to create transgenic organism with mutation in every cell. I created TAL nucleases against eGFP and Drosophila AdoR receptor and combined them with UAS-Gal4 system to test if it is possible to use this genetic tool also for tissue specific mutagenesis.
Anotace v angličtině
-
Klíčová slova
-
Klíčová slova v angličtině
-
Rozsah průvodní práce
76 s.
Jazyk
CZ
Anotace
TAL nucleases are frequently used for mutagenesis in germline to create transgenic organism with mutation in every cell. I created TAL nucleases against eGFP and Drosophila AdoR receptor and combined them with UAS-Gal4 system to test if it is possible to use this genetic tool also for tissue specific mutagenesis.