In this study we focused on detection of galactose-alpha-1,3-galactose (alpha-gal epitope) as to determine the proteins which carry this glycan in ticks. Affinity chromatography with magnetic beads was used to purify the proteins from tick cell lines. The proteins obtained were visualized by SDS-PAGE and western blotting. Purified proteins were then identified using mass spectometry. Fluorescence microscopy was also used to detect Gal as well as another allergenogenic epitope, the core alpha-1,3-fucose, in ticks cells.
Klíčová slova
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Klíčová slova v angličtině
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Rozsah průvodní práce
34 s.
Jazyk
CZ
Anotace
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Anotace v angličtině
In this study we focused on detection of galactose-alpha-1,3-galactose (alpha-gal epitope) as to determine the proteins which carry this glycan in ticks. Affinity chromatography with magnetic beads was used to purify the proteins from tick cell lines. The proteins obtained were visualized by SDS-PAGE and western blotting. Purified proteins were then identified using mass spectometry. Fluorescence microscopy was also used to detect Gal as well as another allergenogenic epitope, the core alpha-1,3-fucose, in ticks cells.