- Pro tuto VŠKP nejsou definovány žádné údaje, u kterých by bylo požadováno jejich vyplnění.
Hlavní téma
The recent technologies in DNA sequencing like nanopore and PacBio allow direct sequencing of long single strands of DNA without amplification. This opens the path to a much improved environmental genome sequencing of bacteria, archaea and viruses. However, our DNA extraction methods have not been tested and updated for these new technologies.
Hlavní téma v angličtině
The recent technologies in DNA sequencing like nanopore and PacBio allow direct sequencing of long single strands of DNA without amplification. This opens the path to a much improved environmental genome sequencing of bacteria, archaea and viruses. However, our DNA extraction methods have not been tested and updated for these new technologies.
Název dle studenta
Optimizing soil nucleic acid extraction protocol to fit third-generation, single-strand sequencing technologies
Název dle studenta v angličtině
Optimizing soil nucleic acid extraction protocol to fit third-generation, single-strand sequencing technologies
Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic acids have yet to keep pace with the requirements of modern third-generation sequencing methods. Therefore, we aim to improve the extraction protocol and create a fast, straightforward, and high-yield purification method for obtaining high-quality nucleic acids.
Anotace v angličtině
Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic acids have yet to keep pace with the requirements of modern third-generation sequencing methods. Therefore, we aim to improve the extraction protocol and create a fast, straightforward, and high-yield purification method for obtaining high-quality nucleic acids.
Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic acids have yet to keep pace with the requirements of modern third-generation sequencing methods. Therefore, we aim to improve the extraction protocol and create a fast, straightforward, and high-yield purification method for obtaining high-quality nucleic acids.
Anotace v angličtině
Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic acids have yet to keep pace with the requirements of modern third-generation sequencing methods. Therefore, we aim to improve the extraction protocol and create a fast, straightforward, and high-yield purification method for obtaining high-quality nucleic acids.
Original protocol, Final version of modified protocol, Equipment and chemicals list
Přílohy vázané v práci
schémata
Převzato z knihovny
Ne
Plný text práce
Přílohy
Posudek(y) oponenta
Hodnocení vedoucího
Záznam průběhu obhajoby
The head of the commission welcome the student and the members of the commission, all members of the commission were present. The student presented the studied protocol of DNA isolation, the aims, methods used and the results.
The supervisor and the opponents presented their reviews, and the student answered the opponents’ questions. Mostly, the student was not able to answer the questions correctly or completely.