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Lecturer(s)
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Course content
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Content of lectures: (1) Plant-microbe interactions. Examples (pathogens and symbiosis) and why it is important to study this topic. Macroscopic outcomes of the interaction. Pathogens lifestyles. Introduction to plant immunity. Comparison of plant and animal immunity. Constitutive and induced type of immunity. A basic history of plant immunity: Two main historical concepts for plant immunity: "Gene for gene" and "zig-zag". (2) TOP10 questions waiting for answers in Molecular Plant-Microbe Interactions. (3) How plant triggers an immune response. Recognition of pathogen (PRRs - pattern recognising receptors; NLRs - nucleotide-binding leucine-rich repeats receptors). (4) Pattern (or PAMP) triggered immunity (PTI). The consequences of such triggered immunity. (5) How microbes infect the plants and suppress plant immunity (especially PTI). What are effectors. Effectors triggered susceptibility (ETS). Hypersensitive response (HR). Plant response to ETS. Effector triggered immunity (ETI). (6) Phytohormonal signalling in plant immunity. The main phytohormonal "triumvirate" (salicylic acid, jasmonic acid, ethylene). The role of other phytohormones. (7) Influence of abiotic stress on plant immunity. The disease triangle. Particular effects of abiotic stresses on plant immunity (temperature, light, others). (8) And what about symbiosis? (there are not only enemies). Types of symbiosis (bacteria, fungi). How is it possible that the plant can distinguish between pathogens and symbionts? (9) Plant microbiome. (10) "Elevator" talk - the lecture full of short students presentations. (11) The knowledge of plant immunity in applications research. (12) "Discussion lecture": Two teams of students will discuss a selected topic. One group will be for, the second against and the students will have to rais their arguments. (13) Possible on-line lecture (MS teams) with abroad expert (my recent candidates are Prof. Silke Robatzek (LMU Munich, Germany); Prof. Kenichi Tsuda (Wuhan University, China) and Dr. Tetiana Kalachova (IEB Prague, CZ). Content of tutorials/seminar: Monday: Introduction to the labs (morning); plant treatment with MAMPs and Pseudomonas syringae (afternoon). Tuesday: Introduction to the method: Callose analysis, collection of the leaves for callose analysis (morning); Introduction to the ROS assay, cut the leave discs for ROS assay, continue in callose analysis (afternoon). Wednesday: ROS assay, seedlings growth inhibition assay (morning); microscopy of the callose (afternoon). Thursday: Introduction to the methods: Pseudomonas assay and genotyping; a collection of the samples from Pseudomonas assay and for genotyping (morning); DNA isolation, PCR, electrophoresis (whole day). Friday: colony counting and analysis of the results from Pseudomonas assay (morning); Presentations of the results (afternoon). Available materials: https://elearning.jcu.cz/course/view.php?id=8537
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Projection, Laboratory, Work with multi-media resources (texts, internet, IT technologies), Group work
- Class attendance
- 26 hours per semester
- Preparation for classes
- 24 hours per semester
- Preparation for credit
- 25 hours per semester
- Preparation for exam
- 25 hours per semester
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Learning outcomes
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The aim of the subject is to introduce students to the topic of plant-microbe interactions with a particular focus on the events that occurred during plant immune responses. We will describe the macroscopic outcomes of plant-microbe interactions but mostly we will focus on the molecular events. We will cover recent discoveries and their consequences for applications (especially for agriculture), which should be students able to discuss, after finishing the course. The subject includes also a tutorial during which the students will learn fundamental techniques used for the study of plant immune responses. In the frame of the subject, I would like to improve the communication skills of the students, which is based on their obligatory to perform two types of presentations, be active in discussion "hour" and pass an oral exam.
In the subject, student should obtain skill to critically discuss topics related to plant immunity (especially on cellular level). During the subject, student will pass discussion with colleagues, ppt presentation and short presentation without ppt. The obtained skills will be also evaluated during the lecture of invited speaker who will present research data connected with plant immunity.
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Prerequisites
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Basic knowledge in plant physiology and in molecular and cell biology. The prerequisites are: Plant Physiology (KEBR 220) or Plant Physiology - Introduction (KEBR 562) and Planc cell biology (KEBR 945).
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Assessment methods and criteria
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Oral examination, Analysis of creative work (musical, visual, literary), Interview, Systematic student observation, Test
To pass the course, students will be evaluated based on their short presentation (so-called "elevator talk"), further based on their activity during the lecture which will be dedicated to discussion about a chosen topic, further perform "classical" presentation using ppt (in pairs) and by attendance of the tutorial. The students will have to pass a combined exam. The evaluation mark consist of evaluation of discussion (20%), elevator talk (20%), ppt presentation (20%), test (20%) and oral exam (20%).
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Recommended literature
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A Color Handbook on Practical Plant Pathology, Vidjay Yanav:https://www.amazon.com/Colour-Handbook-Practical-Plant-Pathology/dp/9385516175.
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Biochemistry and Molecular Biology of Plants, 2nd Edition, Buchanan a kol., kapitola 21: "Responses to plant pathogens": https://www.wiley.com/en-us/Biochemistry+and+Molecular+Biology+of+Plants%2C+2nd+Edition-p-9780470714218.
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Plant Pathology, Agrios, 5th edition:https://www.elsevier.com/books/plant-pathology/agrios/978-0-08-047378-9.
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přednášky (budou k dispozici v Moodle).
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publikace v prestižních časopisech (poskytnuté autorem přednášek).
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