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Lecturer(s)
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Kocábek Tomáš, Ing. Ph.D.
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Course content
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Content of lectures: Bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes and their T-DNA genes, virulence region genes, the mechanism of T-DNA transfer into the plant genome. Plasmid vectors for Agrobacterium-mediated plant transgenosis and direct gene transfer. Selectable genes and reporter genes. Transient expression of transgenes. Virus vectors. Stability of expression of transgenes, gene silencing. Proof of stable integration of T-DNA. Herbicide tolerance introduced into the plant genome by transgenosis. Resistance to pests and pathogens. Transgenes which carry added value into the plant genome. Plant transgenes important for science. Evaluation of environmental and health impacts of transgenes. Legislative aspects of genetically modified organisms. Content of practices: Transformation of plant segments by Agrobacterium tumefaciens in vitro. Detection of transgens by PCR. Protoplast isolation, direct transformation of protoplasts via PEG, transient expression of GUS in protoplasts. Fluorimetric and histochemical assay of GUS.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Experiment
- Preparation for classes
- 10 hours per semester
- Preparation for exam
- 30 hours per semester
- Class attendance
- 50 hours per semester
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Learning outcomes
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The objective of this course is to provide students with an outlook in the current state of the introduction of foreign cloned genes into the plant genome. The principles, results and applications of plant gene transfer will be discussed in connection with the study of possible risks and legislative concerning genetically modified plants.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Combined exam
Active participation in a block exercise, the student will design a brief project to improve a trait in a selected plant species through genetic modification. The oral part of the examination is waived for well prepared projects.
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Recommended literature
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Ondřej, M Drobník, J.: Transgenoze rostlin, Academia Praha 2002.
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Ondřej, M. et al.: Genové inženýrství rostlin. Skriptum, VŠCHT Praha 1999.
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Ondřej, M.: Genové inženýrství kulturních rostlin. Academia, Praha 1992.
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Hruban, V. Hruban, V.: Principy a aplikace molekulární genetiky ve šlechtění. ČZU Praha, 2011. Praha, 2011.
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Vondrejs, V. Vondrejs, V.: Otazníky kolem genového inženýrství. Praha, Academia, 2010. Praha, 2010.
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