|
Lecturer(s)
|
-
Petr Jaroslav, prof. Ing. DrSc.
|
|
Course content
|
The content of lectures: 1) Endocrinological manipulation with animals - control of the sexual cycle, induction of ovulation, embryo transfer, a sampling of oocytes, hormonal growth promoters. 2) In vitro cultivation - in vitro growth and maturation of oocytes, in vitro fertilization, in vitro embryo development. 3) Deep embryo freezing and germ cells - theoretical backgrounds, cryoconservation, classic freezing methods, vitrification. 4) Assisted reproduction -subzonal insemination, intracytoplasmatic sperm injection, embryo biopsy, assisted hatching, the use of polar bodies for embryo production. 5) Biotechnology in males -spermatogonial transfer, in vitro spermatogenesis. 6) Animal cloning, theoretical bases and techniques. The effect of the cell cycle and karyoplast, Roslin technique of cloning, Honolulu technique of cloning, cloning of embryo stem cells and their aggregation with tetraploid embryos. 7) Animal cloning and its practical use. Reproduction and therapeutic cloning. 8) Embryo stem cells, stem cells, cell therapy and tissue engineering. 9) Transgenic animals - methods of application. Microinjection into pronuclei, nuclear transfer, virus vectors, sperms as vectors. 10) Transgenic animals - use in animals. Gene knockout, conditioned gene knockout, transgenic laboratory animals as models. 11) Transgenic animals - application in husbandry. An increase of the growth of farm animals, induction of resistance to infective diseases and stress environmental effects, production of pharmacologically useful proteins, production of qualitatively new animal products, transgenic sows for xenotransplantations, production of new raw materials by means of transgenic animals. 12) DNA vaccines, gene therapy, principles of gene transfer (virus and non-virus vectors), somatic gene therapy, germline gene therapy. 13) Possibilities of saving endangered species by biotechnology. 14) Ethical issues of biotechnology
|
|
Learning activities and teaching methods
|
Monologic (reading, lecture, briefing)
- Class attendance
- 26 hours per semester
- Preparation for exam
- 30 hours per semester
- Preparation for classes
- 30 hours per semester
|
|
Learning outcomes
|
The aim of the course is to give a general overview of the biotechnology of animals, i.e. methods, practical possibilities of animal biotechnology, and bioethical issues.
The student will obtain an overview of various aspects concerning animal biotechnology, transgenic organisms, and IVF.
|
|
Prerequisites
|
Basic understanding of genetics and cell biology.
|
|
Assessment methods and criteria
|
Test
To pass the exam, the student must score at least 60% of points from the final examination test.
|
|
Recommended literature
|
-
Gilbert S.C. et al.: Bioethics and the New Embryology, Sinauer Associates, Sunderland, 2006.
-
Gilbert S.F.: Developmental Biology, 8th Edition, Sinauer Associates, Sunderland, 2006.
-
Neill J.: Physiology of Reproduction, 3rd Edition, Elsevier Academic Press, London, 2006.
-
Renaville R., Burny A.: Biotechnology in Animal Husbandry, Kluwer Academic Publisher, Dordrecht, 2001..
-
Thieman W.J., Palladino M.A.: Introduction to biotechnology. Pearson Edication Inc., San Francisco, 2004.
|