| Course title | Advanced methods of molecular biology |
|---|---|
| Course code | KMB/602E |
| Organizational form of instruction | Lesson |
| Level of course | Doctoral |
| Year of study | not specified |
| Frequency of the course | In each academic year, in the summer semester. |
| Semester | Summer |
| Number of ECTS credits | 4 |
| Language of instruction | English |
| Status of course | Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Course availability | The course is available to visiting students |
| Lecturer(s) |
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| Course content |
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Students select 2-3 of the listed practical sessions: 1. qPCR expression analysis (Adam Bajgar) 2. Advanced Drosophila genetics - generation and analysis of mitotic clones (Tomáš Doležal) 3. Systemic RNA interference in insects (David Doležel) 4. Microinjections and immuno-fluorescence staining of mouse embryos (Alexander Bruce) 5. Fluorescence in situ hybridization (Petr Nguyen) 6. Flow cytometry and cell sorting (Tomáš Korytář) 7. Detection of RNA modifications by afinity gel electrophoresis (Zdeněk Paris) 8. 2D native/SDS electrophoresis combined with immunodetection (Roman Sobotka) 9. Expression and purufication of His-tagged proteins (Roman Sobotka) 10. Classical histological techniques and light microscopy (Hana Sehadová) 11. Detection of high molecular weight respiratory complexes using native PAGE and subsequent Western blot analysis (Alena Zíková) 12. Measurement of mitochondrial respiration by High-Resolution Respirometry (HRR) using the O2k-FluoRespirometer (Eva Doleželová) 13. Western blot in insect tissues and cell lines (Marek Jindra) 14. Next generation sequencing data processing (Petr Nguyen) 15. RNA-seq library preparation and data analysis (Lenka Gahurová, Iva Mozgová) 16. Transfection of insect cell lines (Michal Žurovec)
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| Learning activities and teaching methods |
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Monitoring, Demonstration, Laboratory, Experiment
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| Learning outcomes |
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The aim of the course is to provide practical hands-on experience with selected advanced molecular biology techniques
During the course, students will gain deeper knowledge and understanding about the majority of molecular biology techniques and their applications. Students should be able to independently select optimal methods for solving particular biological problems. They should also be able to advise other students on selection of the best method, and on its advantages and risks. |
| Prerequisites |
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Students should understand principles of molecular biology techniques. They should have basic knowledge from cell biology, molecular biology, developmental biology and genetics.
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| Assessment methods and criteria |
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Student performance assessment, Analysis of student's work activities (technical works)
To pass the practical part of the course, students must attend two selected practical sessions. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Fyziologie a vývojová biologie (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Physiology and Developmental Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Parasitology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Parasitology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Parasitology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Physiology and Developmental Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Physiology and Developmental Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |