|
Lecturer(s)
|
-
Gahurová Lenka, Mgr. Ph.D.
-
Bruce Alexander William, prof. Ph.D.
-
Bohuslavová Martina, Mgr.
-
Collier Rebecca, MSc.
-
Doležal Tomáš, doc. Mgr. Ph.D.
-
Sobotka Roman, prof. Ing. Ph.D.
|
|
Course content
|
Introduction to biology and definition of life. Evolutionary theory and the questions it has raised about heredity and diversity of organisms. The origin of life, the cell as the basic unit of life, the domains of life. Metabolism - cellular energetics and the energy cycle - respiration and photosynthesis. Cellular compartments in terms of metabolism. Mendelian genetics, chromosomal theory of heredity, DNA as a carrier of hereditary information, deciphering the genetic code. Central dogma. Molecular structure of genes and chromosomes: molecular definition of gene, chromosomal arrangement of genes and non-coding DNA, structural arrangement of chromosomes. Basic molecular genetic mechanisms, DNA replication: Understanding the central dogma of molecular biology and review of basic molecular genetic mechanisms. Basic features of DNA replication in vivo. Transcription and RNA processing, translation and genetic code: principles of transcription in prokaryotes and eukaryotes, basic regulation of gene expression at transcriptional and post-transcriptional levels. Principles of translation, genetic code. Mutations, DNA repair and recombination: molecular basis of mutations, induced mutagenesis, overview of repair mechanisms and principles of recombination. Genetic diversity - germ vs. somatic lineage, mitosis/meiosis. Regulation of gene expression in prokaryotes, eukaryotes and genetic control of development: operon, molecular control of transcription in eukaryotes, gene expression and chromosome organization, mechanisms of regulation of gene expression during development. Cellular differentiation and epigenetics. Cell behaviour during development. Evo-Devo. Molecular analysis of genes and gene products: use of recombinant DNA technology to identify genes, molecular diagnosis of human diseases. Recombinant DNA technology, polymerase chain reaction: Cloning of genes, principle of polymerase chain reaction and its applications. Overview of applications of molecular biology in modern basic and applied science: Summary of the subject in the context of the use of molecular biology in modern research.
|
|
Learning activities and teaching methods
|
Monologic (reading, lecture, briefing), Work with text (with textbook, with book)
- Preparation for classes
- 50 hours per semester
- Class attendance
- 20 hours per semester
- Preparation for exam
- 20 hours per semester
|
|
Learning outcomes
|
The subject aims to be an introduction into molecular biology of the cell. Basic molecular biology mechanisms in cells will be reviewed and overview of applications of molecular biology techniques in today's science will be given. Course taught in English.
Students successfully passing this course will have been provided a comprehensive foundation in Molecular & Cellular Biology and Genetics (encompassing theory and the application of basic molecular biology laboratory techniques). This knowledge will be an essential primer for future studies and course modules within the greater program (including providing initial knowledge and competence required for laboratory based practical projects).
|
|
Prerequisites
|
This course represents a fundamental foundation of the subject that will be relied upon in future courses in the program. Consequently it does not require any given prior knowledge but the student should be fully aware that the content learned in this course will form prerequisite knowledge for future courses.
|
|
Assessment methods and criteria
|
Written examination, Test
The "basic pass grade" (3) is 51-59%, grade "good" (2.5) is 60-66%, grade "very good" (2) is 67-74%, grade "excellent minus" (1.5) is 75-82% and the grade "excellent" (1) is 83-100%.
|
|
Recommended literature
|
-
Bruce Alberts et al.,: Molecular Biology of the Cell - Fifth Edition onwards, Taylor & Francis Books Ltd..
-
D. Peter Snustad and Michael J. Simmons: Principles of Genetics, Wiley.
-
David P. Clark: Molecular Biology Understanding the Genetic Revolution, Academic Cell..
-
Harvey Lodish et al.: Molecular Cell Biology - Fifth Edition, W.H. Freeman, 2004.
|