| Course title | Cytogenomics |
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| Course code | KMB/935 |
| Organizational form of instruction | Lecture + Lesson |
| Level of course | Master |
| Year of study | not specified |
| Frequency of the course | V každém akademickém roce, jen v zimním semestru. |
| Semester | Winter |
| Number of ECTS credits | 4 |
| Language of instruction | English |
| Status of course | unspecified |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Content of lectures: 1-2 Chromosome Structure and Function Mitosis, meiosis, anatomy of chromosomes, including structural components, centromeres, telomeres, genome composition and repetitive sequences, banding techniques. 3 Fluorescent In Situ Hybridization (FISH) FISH principles, applications in cytogenetics, advanced FISH techniques, fluorescence microsopy 4 Array Comparative Genomic Hybridization (aCGH) Principles of aCGH, clinical applications in cytogenomics 5-6 Next-Generation Sequencing (NGS) in Cytogenomics Recapitulation of NGS technologies, their impact on understanding genomic variation, epigenomic landscapes, genome composition, Oligopaint 7 3D Nuclear Organization Basics of 3D nuclear organization, chromatin structure, methodologies (e.g., Hi-C) 8 Chromatin and Epigenomics Definitions and basic concepts of epigenetics, including DNA methylation, histone modifications, non-coding RNAs 9-10 Epigenomic Landscapes Exploration of large-scale epigenomic studies, covering techniques such as ChIP-seq (Chromatin Immunoprecipitation sequencing), DNA methylation profiling, ATAC-seq 11-12 Repetitive Sequences Understanding repetitive sequences in the genome, including transposons, satellite DNA, and tandem repeats. Exploration of methodologies for repetitive sequence analysis. 13 Applications of Cytogenomics Cytogenomics in genetic disorders and cancer, personalized medicine Content of tutorials: one week's block of practicals will supplement the lectures and it will include modern molecular cytogenetic methods such as fluorescence in situ hybridization with oligo paint probes and immunodetection of heterochromatin markers.
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| Learning activities and teaching methods |
| unspecified |
| Learning outcomes |
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The Cytogenomics course aims to provide students with a foundational understanding of modern cytogenetic and cytogenomic approaches. It explores advanced genomic technologies and their applications chromosome biology studies of, 3D nuclear organization, epigenetics, and genome composition. Students will learn how are cytogenomic techniques applied in diagnosing genetic disorders and cancer.
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| Prerequisites |
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unspecified
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| Assessment methods and criteria |
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unspecified
Credit: attendance at the practicals, presentation of selected applications and case studies in cytogenomics Exam: oral and written exam (min. 50%) |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester |
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