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Lecturer(s)
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Jalovecká Marie, RNDr. Ph.D.
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Fiala Ivan, RNDr. Ph.D.
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Kamiš Jan, RNDr.
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Course content
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Content of lectures: 1. Kingdoms of organisms, protista vs. protozoa: definition of Protozoa. Origin of protists and the "Tree of life". Adaptations of protozoa to parasitism. Protozoan phyla and classification. 2.-12. A detailed description of individual groups of parasitic protozoa with emphasis on knowledge of life cycles, cells and its properties/adaptation, medical or veterinary significance and introduction to basic methods of detection, therapy and prevention. Groups of parasitic organisms that will be introduced in the course: Alveolata (Dinoflagelata, Apicomplexa, Ciliata - talks 2+3+4+5), Discoba (talks 6+7), Metamonada (talks 8+9), Amobeozoa (talk 10), Microsporidia, Myxozoa (talk 11), mall groups of parasites from Rhizaria, Archaeplastida, Stramenopila a Fungi (talk 12). Discussion: recent opinions on the systematics of protists and development of therapeutics or vaccines. "Journal Club" about the most recent knowledge of important protozoans or advances in the field. Content of practices: Safety measures in handling parasitic protists. Demonstration of representative parasitic organisms of the individual groups including special preparation techniques.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, Laboratory, Experiment, Individual tutoring
- Preparation for classes
- 30 hours per semester
- Class attendance
- 90 hours per semester
- Preparation for credit
- 20 hours per semester
- Preparation for exam
- 100 hours per semester
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Learning outcomes
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To provide students with key features of the most important groups of parasitic protists: Alveolata (Dinoflagelata, Apicomplexa, Ciliata), Stramenopila, Discoba, Metamonada, Amobeozoa, Microsporidia, Myxozoa + small groups of parasites from Rhizaria, Archaeplastida and Fungi (using the Tree of life model). The knowledge of host cell structure, adaptation to a parasitic way of life, life cycles including parasite-host relationships, and medical or veterinary importance together with diagnostics, prevention and therapy in all groups. Practical knowledge of the most important parasites - recognition of parasites on microscopic slides or in vivo in cultures.
Knowledge of systematic classification of individual groups of parasitic protists using the "Tree of life" model. Knowledge of basic characteristics of these groups - cell organization, adaptation to a parasitic way of life. Knowledge of parasitic protists variability and their relationship with the host. Knowledge of life cycles of the most important parasitic agents and their medical or veterinary importance. Knowledge of basic parasitological diagnostics incl. the most commonly used methods of detection, therapy and prevention. Practical knowledge of the most important parasites based on microscopic observation.
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Prerequisites
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The course requires basic knowledge of cell and molecular biology (e.g. KBI / BIO01 course). Knowledge of invertebrate zoology or general protistology is an advantage (e.g. courses KZO / 155 or KZO / 161). Students are encouraged to enroll in the course Biology of Parasitism (KPA / 170) and Biochemistry and Mol. biol. of parasites (KMB / 487). To obtain deep knowledge of parasitic organisms, the enrollment in the courses Parasitic Arthropod Biology (KPA / 486) and Helminth Biology (KPA / 484) is recommended. Special course of master's or doctoral study program Parasitology, suitable also for students of master's or doctoral study program Molecular Biology using protozoa as model organisms.
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Assessment methods and criteria
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Combined exam, Colloquium, Test
To successfully complete the course students are required to: 1. Actively participate and present in the scope of the "Journal Club". 2. Participate in exercises and recognize typical representatives of parasitic protozoa - "zápočtový test" 3. Prove the knowledge of the course subject during the oral examination.
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Recommended literature
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Archibald, John M., Simpson, Alastair G.B., Slamovits, Claudio H. (Eds.). Handbook of the Protists. Springer, 2017. ISBN 978-3-319-28147-6.
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David P. Hughes, Jacques Brodeur, Frédéric Thomas. Host manipulation by parasites. Oxford, 2012. ISBN 978-0-19-964223-6.
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Denis H. Lynn. The ciliated protozoa : characterization, classification, and guide to the literature. Springer, 2008. ISBN 978-1-4020-8238-2.
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Iva Dyková and Jiří Lom. Histopathology of protistan and myxozoan infections in fishes : an atlas. Academia, 2007. ISBN 978-80-200-1546-4.
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Jan Votýpka, Iva Kolářová, Petr Horák a kol. O parazitech a lidech. Triton, 2018. ISBN 978-80-7553-350-0.
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Jírovec, Otto a kol. Parasitologie pro lékaře. Avicenum, 1977.
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John J. Lee and kol. Illustrated Guide To The Protozoa. ALLEN PRESS, 2000. ISBN 9781891276224.
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Klaus Hausmann, Norbert Hülsmann. Protozoologie. Academia, 2003. ISBN 80-200-0978-7.
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Petr Volf, Petr Horák. Paraziti a jejich biologie. Triton, 2007. ISBN 978-80-7387-088-9.
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Rosypal, Stanislav. Nový přehled biologie. Scientia, 2003. ISBN 978-80-86960-23-4.
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