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Lecturer(s)
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Franta Zdeněk, RNDr. Ph.D.
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Fessl Tomáš, Mgr. Ph.D.
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Dyčka Filip, Mgr. Ph.D.
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Course content
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Content of lectures: 1. Introduction to biochemical and molecular-biological studies (equipment and safety in the laboratory, GLP, experiment design, nucleic acids: structure and function, genes and genome, isolation and work with NA, PCR, sequencing, basics of bioinformatics) 2. Methods and use of recombinant DNA in biology (cloning of genes and cloning vectors, gene analysis and expression, expression of foreign genes and preparation of recombinant proteins, types of expression systems basics of cell cultures) 3. Proteins (protein function and structure, posttranslational modification, (enzymes: active sites and catalytic mechanism, control of enzymatic activity, utilization of enzymes), protein engineering, proteomics) 4. Electrophoretic methods (principles, media, agarose Electrophoresis (DNA, RNA and capillary electrophoresis) SDS-PAGE, native gels, gradient gels, 2D gels, zymography, Western blotting, immunobiochemistry) 5. Chromatography (Principles, Types of Chromatography, HPLC, Ion, Affinity, SEC, Adsorption, Distributing, Gas) 6. Centrifugation (principle and types of centrifuges, rotors, sedimentation principles, preparative centrifugation, analytical centrifugation) 7. Microscopy (introduction and types of microscopes, composition of microscopes, optical microscopy (techniques and limitations, resolution, sample preparation, stereomicroscope, confocal microscopy, fluorescence microscopy). Electron microscopy (types and techniques, preparation of samples, limitations), visualization in living cells and tissues 8. Mass spectrometry techniques 9. Spectroscopic methods in biochemistry Content of practicals: During the course the knowledge gained during the lectures will be enhanced.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, Laboratory, Practical training
- Class attendance
- 26 hours per semester
- Preparation for exam
- 25 hours per semester
- Preparation for classes
- 50 hours per semester
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Learning outcomes
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The lectures provide students with biochemical methods to monitor cell life, and thus the function of countless proteins and other macromolecules, before structurally describing very complex molecules of enzymes, receptors, transporters, regulators, nucleic acids.
Student should understanding basic principles on techniques in molecular biology a biochemistry according to the specific lectures in the section "Obsah, opory".
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Prerequisites
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Basic knowlegde in biology, biochemistry and chmistry.
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Assessment methods and criteria
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Student performance assessment
Active understanding of the subject. The progress is continuously checked during the course by group discussions. It is required min. 60 % in the final exam.
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Recommended literature
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Bruce Alberts, Dennis Bray, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter: Základy buněčné biologie: úvod do molekulární biologie buňky, původně Essential Cell Biology. Second Edition. New York: Garland Science. ISBN: 0-8153-3480-X (hc); 0-8153-3481-8 (pb), 2004..
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https://www.databazeknih.cz/knihy/zaklady-bunecne-biologie-uvod-do-molekularni-biologie-bunky-45040.
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ŠMARDA, Jan. Metody molekulární biologie. 1. vyd. Brno: Masarykova univerzita, 2005, 188 s. ISBN 80-210-3841-1..
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Wilson, Keith, and Walker, John (eds.): Principles and techniques of biochemistry and molecular biology (6th Ed.) Cambridge University Press, Cambridge, 2005, 802 pp., ISBN 0-521-82889-9,P70.00.
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