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Lecturer(s)
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Khanna Ritesh
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Mašková Hana, Mgr.
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Selinger Martin, RNDr. Ph.D.
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Tučková Dominika, Ing.
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Frejlachová Andrea, Mgr.
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Doudová Lenka, Mgr.
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Jaklová Kateřina, Mgr.
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Dvorníková Zuzana, Mgr.
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Tykalová Hana, Mgr. Ph.D.
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Hejduk Libor, Mgr.
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Course content
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Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification. Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography. DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA. Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction. SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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Learning activities and teaching methods
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Skills training, Laboratory
- Class attendance
- 42 hours per semester
- Preparation for credit
- 8 hours per semester
- Preparation for classes
- 8 hours per semester
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Learning outcomes
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The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
Learning of the basic methods in biochemistry
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Prerequisites
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Povinná prerekvizita pro studenty Biological Chemistry: Laboratory Course in General Chemistry (JKU, Linz); or the courses Introductory Lab Course and the Lab Course in Analytical Chemistry.
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Assessment methods and criteria
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Student performance assessment, Test
Active work in the laboratory, theoretical preparation for laboratory tasks (tested before laboratory), 100% participation in all laboratory tasks and work on the assignments (results calculation from the measurements), and obtaining 60% of points in the tests.
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Recommended literature
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