|
Lecturer(s)
|
-
Štěrba Ján, RNDr. Ph.D.
-
Štěrbová Jarmila, RNDr.
-
Věchtová Pavlína, RNDr. Ph.D.
|
|
Course content
|
Content of lectures: 1. Posttranslational modifications of proteins. Introduction to glycobiology. Glycans, glycoproteins, lectins, and their importance. 2. Glycosylation. N-glycans, their importance in proteosynthesis. 3. O-glycans, other glycan types. 4. N-glycan biosynthesis in Eukaryotes and bacteria, its localization in the cell. 5. Glycosyltransferases and glycosidases. 6. Differences in glycosylation - glycosylation in mammals, arthropods, plants, parasites, and bacteria. 7. Clinical aspects of glycosylation, sialic acid, fucose, galactose - glycans and infection, inherited glycosylation disorders, allergic reactions, transplantation. 8. Clinical aspects of glycosylation, sialic acid, fucose, galactose - allergic reactions, glycans as disease markers. 9. Lectins and saccharide/glycans-specific antibodies. Galectins, C-lectins, P-lectins, etc. 10. Glycomics, glycoproteomics - the use of lectins and antibodies. Structural analysis, chemical and enzymatic synthesis. Glycosylation inhibitors. Glycan-labelling approaches. 11. Glycomics, glycoproteomics - chemical and enzymatic synthesis. Glycosylation inhibitors. Glycan-labelling approaches. 12. Glycobiology in biotechnology - production of recombinant glycoproteins and problems related to their use. Examples.
|
|
Learning activities and teaching methods
|
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming)
- Class attendance
- 30 hours per semester
- Preparation for classes
- 25 hours per semester
- Preparation for exam
- 20 hours per semester
|
|
Learning outcomes
|
Lectures will introduce the students to the world of posttranlastional modifications of proteins, primarily glycosylation. The different glycosylation types will be explained as well as their biosynthesis, differences in glycan structures between various organisms and importance of these differences from the medicinal point of view. Lastly, methods for glycans and glycoproteins analyses will be shown.
Basic knowledge in glycobiology and glycobiochemistry will be provided
|
|
Prerequisites
|
Biochemistry, Mol. and Cellular Biology
|
|
Assessment methods and criteria
|
Interview, Combined exam
Students must correctly answer at least 50 % of test questions.
|
|
Recommended literature
|
-
Essentials of Glycobiology, 2nd edition, Editors: Ajit Varki, Richard D. Cummings, Jeffrey D. Esko, Hudson H. Freeze, Pamela Stanley, Carolyn R. Bertozzi, Gerald W. Hart, Marilynn E. Etzler. Cold Spring Harbor Laboratory Press; 2009..
-
Glycobiology, Clare Sansom, Ofer Markoman, Scion Publishing, 2007.
-
The Sugar Code, Editor: Hans-Joachim Gabius, Wiley-Blackwell, 2009.
|