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Lecturer(s)
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Hořejší Karel, Mgr.
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Kahoun David, Ing. Ph.D.
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Course content
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Content of practicals: Two complete procedures using liquid chromatograph coupled to mass detector (LC/MS) and gas chromatograph coupled to mass detector (GC/MS). Each procedure covers: - analytical method optimization - sample storage and handling - sample homogenization - analyte extraction - extract purification - analyte preconcentration - instrumental chemical analysis - data evaluation - results presentation
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Learning activities and teaching methods
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Demonstration, Laboratory
- Class attendance
- 32 hours per semester
- Preparation for classes
- 10 hours per semester
- Preparation for credit
- 15 hours per semester
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Learning outcomes
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To acquire practical knowledge of common procedures which are performed during analyses of real samples using chromatographic methods with mass spectrometry detection.
Student will acquire basic knowledge of complete procedure for real sample analysis, which covers all steps from sample preparation to quantitative analysis, including method optimization.
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Prerequisites
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Basic knowledge of principles of gas chromatography, liquid chromatography and mass detection (target analysis). Good knowlegde of chemical nomenclature and chemical calculations.
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Assessment methods and criteria
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Development of laboratory protocols
To pass the course, student must perform all laboratory exercises and hand in all laboratory protocols worked out in sufficient quality.
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Recommended literature
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HOLČAPEK, Michal. Hmotnostní spektrometrie v organické analýze. holcapek.upce.cz [on-line]. 2015 [cit. 2015-09-07]. Dostupné z: http://holcapek.upce.cz/vyuka-ms-org-anal.php.
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CHURÁČEK, Jaroslav. Analytická separace látek. 1. vyd. Praha: Státní nakladatelství technické literatury, 1990. 384 s. ISBN 80-03-00569-8.
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NOVÁKOVÁ, Lucie a Michal DOUŠA. Moderní HPLC.
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NOVÁKOVÁ, Lucie a Michal DOUŠA. Moderní HPLC separace v teorii a praxi I. 1. vyd. Praha: Lucie Nováková, 2013, 299 s. ISBN 978-80-260-4243-31.
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