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Lecturer(s)
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Kopecký Jiří, Ing. CSc.
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Masojídek Jiří, prof. RNDr. CSc.
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Kopecký Jiří
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Course content
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1. Introduction - Biotechnology concepts and trends - overview - Masojídek 2. Biotechnology topics (enzymology, plant biotechnology, cell cultures, tissue cultures, GMO) - overview - Masojídek 3. Fields of Biotechnology (food industry, industrial production, environmental protection, bio-fuels, agriculture and forestry, medicine and pharmaceutical industry) - overview - Kopecký 4. Biotechnology equipment (fermenters, disintegrators, centrifuges, driers, coolers, extractors, storage of products) - Sergejevová 5. Basic types of cultivation processes (batch, pulse, semi-continuous and continuous) - Sergejevová 6. Biotechnology of autotrophic microorganisms - Masojídek - Autotrophic microorganisms taxonomy - photosynthetic bacteria, cyanobacteria, algae (biochemistry and physiology, photosynthesis) - Important production strains (cultivation techniques and conditions) - Cultivation equipment - photobioreactors (laboratory, large-scale, heterotrophic cultivation) - Large-scale production of microalgae (biological principles, most frequently used strains, economy) 7. Basic analytical, biochemical and biophysical methods used in algal biotechnology - Kopecký 8. Bioactive compounds from algae and cyanobacteria - Kopecký 9. Heterotrophic and mixotrophic cultivation of microalgae - Sergejevová 10. Microalgae utilizations - aquaculture, agriculture, hydrogen production, waste-water treatment, bioremediation - Sergejevová 11. Future trends in Biotechnology - GMO, biosensors, etc. - Masojídek 12. Intellectual property protection in biotechnology - Kopecký
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Monitoring, Projection, Laboratory
- Class attendance
- 39 hours per semester
- Preparation for credit
- 3 hours per semester
- Preparation for exam
- 6 hours per semester
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Learning outcomes
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A student will obtain general overview of development, equipment, methodology and current use of biotechnological processes. He/she will be informed about a use of biotechnology in various topics of human activity (nutrition, pharmacology, chemical industry, cosmetics, agriculture, environmental technology). The student will be acquainted with function and use of some equipment (e.g. cultivation unit, microscop, spectrophotometer, centrifuge, measurement of oxygen production and irradiance intensity). The course is focused to microalgal biotechnology.
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Prerequisites
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A student has graduated in natural or technical science. He/she has acceptable knowledge (exam) or passed required credited courses at IPB: Selected parts of chemistry (CHE), Introduction to biochemistry (BCH), Basic of experimental biological methods (ZBE).
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Assessment methods and criteria
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Essay, Analysis of student's language skills, Analysis of student's work activities (technical works), Interview, Questionnaire, Colloquium
Deliver short oral presentation and discuss selected topic. Carry-out practical laboratory experiments aimed to microbiology and biochemistry. Evaluate and discuss results.
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Recommended literature
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www.wikipedia.com.
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Andersen, R.A. Algal Culturing Techniques. Elsevier Academic press, Amsterdam, 2005. ISBN 0-12-088426-7.
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Atkinson, B., Mavituna, F. Biochemical Engineering and Biotechnology Handbook. London, Stockton Press, 1991.
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Kojima et al. Photosynthetic Microorganisms in Environmental Biotechnology. Springer, 2001. ISBN 962-430-136-0.
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Michalík a kol. Biochemické technologie. Nitra, Slovenská poĺnohospodárská univerzita, 1999.
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Richmond, A. Handbook of Microalgal Culture. Blackwell Science, 2004. ISBN 0-632-05953-2.
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Šilhánková, L. Mikrobiologie pro potravináře a biotechnology. 3. přepracované vyd., Praha Academia, 263 s., 2002. ISBN 8-85606-71-6.
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Van den Hoek et al. Algae. Cambridge University Press, 2002. ISBN 0-521-31687-1.
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Vodrážka, Z. Biotechnologie. VŠCHT Praha, 1988. ISBN 80-7080-121-2.
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