| Course title | Optical Spectroscopy |
|---|---|
| Course code | UFY/455 |
| Organizational form of instruction | Lecture + Practice |
| Level of course | Master |
| Year of study | not specified |
| Frequency of the course | In each academic year, in the summer semester. |
| Semester | Summer |
| Number of ECTS credits | 6 |
| Language of instruction | Czech |
| Status of course | Compulsory, Compulsory-optional |
| Form of instruction | unspecified |
| Work placements | unspecified |
| Recommended optional programme components | None |
| Course availability | The course is available to visiting students |
| Lecturer(s) |
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| Course content |
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The course focuses on introducing optical spectroscopy methods, including the theoretical foundation of individual methods and their application, particularly for studying biological and biochemical systems. Lectures are supplemented with practical exercises demonstrating the use of individual methods. Content: 1. Radiation, light-matter interaction, absorption, emission, scattering 2. Radiation sources, detection methods, principles, applications 3. Methods based on light scattering by macromolecules, DLS 4. Absorption spectroscopy 5. Linear and circular dichroism 6. Fluorescence 1, fundamentals of the method: excitation emission spectra, fluorophores, FRET 7. Fluorescence 2, time-resolved fluorescence, fluorescence correlation spectroscopy 8. Vibrational methods, introduction 9. Infrared spectroscopy, FTIR 10. Raman spectroscopy methods, resonance Raman spectroscopy 11. Ultrafast spectroscopy: transient absorption, photon echo, two-dimensional spectroscopy (optional) 12. Optical microscopy, confocal microscopy, localization, high resolution
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| Learning activities and teaching methods |
Monologic (reading, lecture, briefing), Demonstration, Laboratory
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| Learning outcomes |
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This course introduces methods of optical spectroscopy used primarily for the study of organic molecules and biological systems. Lecturer will demonstrate the theoretical fundamentals and the methods were applied at limit frontier of theoretical availability. Instrumentation is involved in practicals.
Students intensify their knowledge of optics, optical instrumentation techniques and methods used primarily for biological and biochemical materials. |
| Prerequisites |
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Basic knowledge of Physics III topics (wave and particle nature of light, interference and diffraction, wave theory, refraction index and polarization)
UFY/CV013 ----- or ----- UFY/437 |
| Assessment methods and criteria |
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Oral examination, Development of laboratory protocols
Part of the course Optical Spectroscopy is a regular, one-week, practice. All protocols on practices are required. Oral exam. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester |
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