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Lecturer(s)
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Bárta Jiří, doc. Ing. Ph.D.
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Course content
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Content of lectures: 1. Specifity of DNA/RNA isolation from soil and water ecosystems 2. PCR amplification of spec. regions DNA of Bacteria and Archaea 3. Online databases (RDP, GenBank, SILVA) 4. DGGE/TGGE - fingerprinting of microbial communities 5. qPCR -quantitative PCR (absolute quantification of selected genes) 6. EEA - extracellular enzyme activities - connection with decomposition of organic matter Content of practicals 1. DNA/RNA isolation from soil and water ecosystems 2. PCR amplification of spec. regions DNA of Bacteria and Archaea 3. DGGE - fingerprinting of microbial communities 4. qPCR -quantitative PCR (absolute quantification of selected genes) 5. EEA - measuring of extracellular enzyme using MUP-substratets
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Practical training
- Preparation for classes
- 20 hours per semester
- Semestral paper
- 20 hours per semester
- Preparation for exam
- 20 hours per semester
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Learning outcomes
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Objective of the curse is to introduce advanced molecular methods which help to study the huge quantity and diversity of microbial word in different environments
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Prerequisites
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knowledge of basic genetics and molecular biology
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Assessment methods and criteria
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Test, Development of laboratory protocols
basic genetic and molecular biology courses biology of microorganisms
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Recommended literature
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Brock: Biology of Microorganisms 12. nebo 13. vydání (Madigan, Martinko, Dunlap, Clark).
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Modern Soil Microbiology, 2. Edice (van Elsas, Jansson, Trevors).
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R studio.
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Arthur Lesk. Introduction to Bioinformatics. ISBN 0199651566.
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