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Lecturer(s)
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Šmilauerová Marie, doc. RNDr. Ph.D.
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Švancárová Tereza, Mgr.
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Kahounová Ludmila, Mgr.
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Klementová Šárka, doc. RNDr. CSc.
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Janíková Eva, Mgr. Ph.D.
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Kaštovský Jan, prof. RNDr. Ph.D.
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Koutecký Petr, Mgr. Ph.D.
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Poncarová Martina, Mgr. et Mgr.
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Robovský Jan, RNDr. Ph.D.
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Duda Pavel, RNDr. Ph.D.
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Kamiš Jan, RNDr.
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Konečná Marie, Mgr.
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Kvičerová Jana, MVDr. Ph.D.
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Manukjanová Alžběta, Mgr. Ph.D.
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Piálková Radka, RNDr. Ph.D.
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Uhrová Michaela, Mgr.
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Course content
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Content of the exercise: Microcopy Theory - Physical foundations of microscopy, types of microscopes. Light microscope construction, microscopy procedure. Properties of microscopic objects, microscopic preparations. Documentation of microscopic objects. Measuring and counting microscopic objects. Contrast enhancement methods. The tasks are focused on practicing the material discussed (working with a stereomicroscope and a classical microscope, adjusting Koehler lighting, documentation, simple staining, microscopy with immersion, comparison of plant and animal tissues). Molecular biology Polymerase chain reaction and electrophoresis Phylogenetic analysis Basics of working with DNA sequences, alignment, interpretation of the phylogenetic tree Work in a chemical laboratory Familiarization with laboratory tools in the workbench, weighing, filtration, centrifugation Preparation of a solution with a precise concentration (standard solution) Acid-base titration for indicator - determination of the exact concentration of a monoacid solution Working with a micropipette Preparation of acid solutions, pH measurement. Preparation of an acid solution with a certain concentration in percent from a stock acid solution Preparation of acid solutions with certain concentrations in moles/l Determination of pH using indicator papers Basics of measuring with a pH meter - the need for calibration, correct handling of the electrode. Demonstration of ions in small concentrations using the formation of colored complexes - working with solutions with small concentrations, correct and precise dilution of solutions. Comparison of the sensitivity of two methods using the formation of colored complexes of metal ions. Getting to know the basics of spectrophotometer measurements. Seminar: Safety of work in a chemical laboratory. Laboratory equipment and laboratory procedures. Basic chemical calculations: Specifying the amount of substances in a homogeneous system - mass, volume, amount of substance (meaning of the term mol and options its use - mass, energy, charge), in the mixture - concentration (mass percent, volume, molar concentration, molar fraction Calculations of percentage concentrations and calculations of changes when diluting solutions/adding components, mixing solutions Conversion of percentage and molar concentrations for acids Calculations of the pH of strong acids (including very dilute solutions) Calculations for the preparation of cultivation media
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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The aim of the course is to learn the basics of working in biological, molecular biological and chemical laboratories, to handle stereomicroscopes and microscopes, to learn the basics of working with the DNA sequences, and basic chemical calculations. The student will be able to use these skills in the following specialized courses.
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Prerequisites
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Highschool level biology and chemistry
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Assessment methods and criteria
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unspecified
The student must get at least 70% examination test score, submission of protocols, attendance, microscope adjustment
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Recommended literature
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Klementová Š., (2015) Základní zásady chování a práce v chemické laboratoři (PřF JU).
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Klementová Š., (2021) Laboratorní praktikum - návody k úlohám (PřF JU)..
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Slonim D. (2003) Optický mikroskop, základní údaje. Biologická fakulta Jihočeské univerzity v Českých Budějovicích.
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Zima J., Macholán M., Munclinger P., Piálek J. (2004) Genetické metody v zoologii. PřF UK, Praha.
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