Course: Genomics

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Course title Genomics
Course code KMB/919
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 6
Language of instruction English
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Horák Aleš, Mgr. Ph.D.
Course content
Content of lectures: 1. Overview of what is genomics, basic genome structure and features, organellar vs. nuclear genomes, viral vs. prokaryotic vs. eukaryotic genomes 2. Genomic databases. General vs. specific, data downloading, genome browsers 3. Genome evolution. Factors affecting genome structure, stabilizing vs. destabilizing forces, coding capacity, C-value paradox 4. Towards your own genome I. How to prepare genomic project, Sequencing platforms, Single cell genomics 5. Towards your own genome II. Read processing, QC, assembling strategies and assemblers, read quantification and mapping 6. Genome annotation. Structural annotation: gene prediction in prokaryotes and eukaryotes, repeats identification. Functional annotation: Blast, PFAM, COG/GO calling, GO Enrichment test. 7. Genome and metabolism. Mapping of genes and transcripts to biochemical pathways, KEGG maps, 8. Cytogenomics. Chromosome structure, gene and chromosome duplications, sex chromosomes vs. Autosomes 9. Meta-omics I. Metabarcoding vs. Metagenomics, amplicon sequencing, prokaryotes vs, eukaryotes, QC and amplicon linking, reference databases, taxonomic binning, alpha/beta diversity 10. Meta-omics II. Metagenomics, metatranscriptomics, taxonomic binning, 11. Phylogenomics. Basic phylogenetic concepts, phylogenetics vs. phylogeno/transcriptomics, dataset compilation, contamination and paralog discovery and removal, LGT detection. Alignment and phylogenomic SW 12. Population Genomics. Future of genomics.

Learning activities and teaching methods
unspecified
Learning outcomes
The course aims to introduce students to the dynamic field of Genomics. They will learn about the genome structure and evolution of the genome and will be familiarised with current methodology that will enable them to plan, start and successfully complete genomic project, including genome assembly and annotation.

Prerequisites


Assessment methods and criteria
unspecified
credit: practicals attendance (max. 3 absences), seminar project exam: final exam (min. 50 %)
Recommended literature
  • Hartwell, Goldberg, Fischer, Hood. Genetics From Genes to Genomes 6th Edition. McGraw Hill, 2018.
  • Lesk. Introduction to Genomics, 2nd edition, Oxford University Press, 2012.
  • Pevsner. Bioinformatics and Functional Genomics 3rd Edition, 2015 Brown. Genomes, Garland Science, 2017.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester