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Lecturer(s)
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Krynická Vendula, Mgr. Ph.D.
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Linhartová Markéta, Mgr. Ph.D.
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Tomanová Lenka, Mgr. Ph.D.
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Skotnicová Petra, Mgr.
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Course content
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Ecophysiology of cyanobacteria: Evolution, distribution, and biodiversity of cyanobacteria, various environmental conditions, structural determinants of cyanobacterial cells (Vendula Krynická) Organization of photosynthetic apparatus, light harvesting and energy flow, comparison of the mechanism of photosynthesis in phototrophic organisms (Markéta Linhartová) Membrane systems in cyanobacteria, outer, plasma, and thylakoid membranes, membrane compartments, architecture of thylakoid membrane, membrane lipids (Lenka Bučinská) Model cyanobacteria and cyanobacterial strains utilized for biotechnology (Lenka Bučinská) Primary carbon and nitrogen metabolism in cyanobacteria, nitrogen fixation (Vendula Krynická) Cell signalling in cyanobacteria, two component systems, transcription factors (sigma factors, repressors), manipulating of regulatory pathways for biotechnological application (Markéta Linhartová) Molecular mechanisms of stress tolerance in cyanobacteria (nutrient UV, HL stress responses and regulatory pathways), general survival mechanisms under stress conditions, storage biopolymers, survival program and its application (Vendula Krynická) Molecular taxonomy of cyanobacteria (DNA, proteins, metabolites, sequencing, genetic analysis of cyanobacteria), cyanobacterial genomes: Geneious and Artemis software packages, understanding gene annotation determined from transcriptional data (Petra Skotnicová) Genetic manipulation of cyanobacteria: transformation, recombination, directed mutagenesis, combinatorial mutagenesis, gene inactivation, Gibbson assembly, Quick change, CRISPR-based mutagenesis (Petra Skotnicová) Omics in cyanobacteria: Genome-wide sequencing, proteomics, transcriptomics and metabolomics. Adaptable laboratory evolution and its use in biotechnology, suppressor mutations (Vendula Krynická) Biotechnology in practice: biotechnological potential of cyanobacteria, production of biofuels and other types of high-value compounds (Vendula Krynická). Secondary metabolites in cyanobacteria, bioactive cyanobacterial peptides with anti-virulent and antiproliferative effect and its use in biotechnology (Vendula Krynická in collaboration with Pavel Hrouzek) In Třeboň, Algatech Centre: Microbiological and molecular biology work with cyanobacteria: Inoculation, re-plating, cultivation, harvesting, transformation, isolation of DNA, isolation of thylakoid membranes, separation of membrane complexes on the native gel, localization of tagged proteins by confocal microscopy.
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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The course " Molecular Biology & Biotechnology of Cyanobacteria " will target students who would like to deal with the use of cyanobacteria in modern biotechnology in the future The course will be led by molecular biologists with many years of experience in the physiology, morphology, mutagenesis of cyanobacteria. General knowledge will be combined with practical applications in biotechnology. In addition to physiology, morphology, the lecturers will introduce signal and regulatory pathways of cyanobacteria. They will explain the methods used for mutagenesis of these pathways and its use in the production of valuable compounds. They will also discuss omic analyses (Whole genome sequencing, proteomic and transcriptomic analysis) and their use in biotechnology. At some lessons, students will be given short homework that should lead them to reflect on the problem discussed in class. In addition to the theoretical classes, the course will include laboratory practise where they will experience the modern methods used in cyanobacterial molecular biology.
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Prerequisites
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Recommended lectures: Practical Computing for Biologists, Introduction to Omics & Biotechnology, Practicals in Omics & Biotechnology, Cell Structure and Function, Molecular Physiology and Metabolism, Gene & Protein Engineering
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Assessment methods and criteria
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unspecified
credit: attendance at practicals exam: oral/written (min. 50 %)
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Recommended literature
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Brett Neilan, Michel Rodrigo Zambrano Passarini, Prashant Kumar Singh, Ajay Kumar, Cyanobacterial Biotechnology in the 21st Century, ISBN-13: 9789819901807.
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Cohen, Y., Gurevitz, M. (2006). The Cyanobacteria-Ecology, Physiology and Molecular Genetics. In: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, KH., Stackebrandt, E. (eds) The Prokaryotes. Springer, New York, NY. DOI:.
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Herrero, A., and Flores, E. (2008). The Cyanobacteria: Molecular Biology, Genomics and Evolution, ISBN 978-1-904455-.
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Los, D. (2017). Cyanobacteria: Omics and Manipulation. DOI: https://doi.org/10.21775/9781910190555, ISBN: 978-1910190-55-5.
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Los, D. (2018). Cyanobacteria: Signaling and Regulation Systems, DOI: https://doi.org/10.21775/9781910190876, ISBN: 978-1-910190-88-3.
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Rajesh Prasad Rastogi (2022), Ecophysiology and Biochemistry of cyanobacteria, DOI: https://doi.org/10.1007/978981-16-4873-1, ISBN978-981-16-4873-1.
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