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Lecturer(s)
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Nguyen Petr, RNDr. Ph.D.
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Gahurová Lenka, Mgr. Ph.D.
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Moos Martin, Ing. Ph.D.
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Chodáková Lenka, Mgr. Ph.D.
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Course content
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Content of tutorials/seminar: Genomics - library preparation, genome sequencing using nanopore technology, data analysis (3 days) Transcriptomics - RNA isolation, library preparation and sequencing using Illumina, data analysis (3 days) Proteomics - proteomics facility tour and proteomics data analysis (1 day) Metabolomics - metabolomics facility tour and metabolomics data analysis (1 day) CRISPR-Cas9 yeast genome editing - gRNA design, plasmid cloning and yeast transformation, phenotype analysis (4 days)
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Learning activities and teaching methods
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- Class attendance
- 50 hours per semester
- Preparation for credit
- 25 hours per semester
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Learning outcomes
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These are 5 block practicals in the last 3 weeks of the semester covering the most commonly used OMICs approaches and gene editing. Three of the multi-day practicals are truly hands-on, two are guided visits to service facilities with the opportunity to try out data analysis.
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Prerequisites
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unspecified
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Assessment methods and criteria
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unspecified
Credit: attendance and approved protocols from the practicals
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Recommended literature
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Detailed protocols for practicals.
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