|
Lecturer(s)
|
-
Nguyen Petr, RNDr. Ph.D.
-
Gahurová Lenka, Mgr. Ph.D.
-
Moos Martin, Ing. Ph.D.
-
Chodáková Lenka, Mgr. Ph.D.
|
|
Course content
|
Content of tutorials/seminar: Genomics - library preparation, genome sequencing using nanopore technology, data analysis (3 days) Transcriptomics - RNA isolation, library preparation and sequencing using Illumina, data analysis (3 days) Proteomics - proteomics facility tour and proteomics data analysis (1 day) Metabolomics - metabolomics facility tour and metabolomics data analysis (1 day) CRISPR-Cas9 yeast genome editing - gRNA design, plasmid cloning and yeast transformation, phenotype analysis (4 days)
|
|
Learning activities and teaching methods
|
|
unspecified
|
|
Learning outcomes
|
These are 5 block practicals in the last 3 weeks of the semester covering the most commonly used OMICs approaches and gene editing. Three of the multi-day practicals are truly hands-on, two are guided visits to service facilities with the opportunity to try out data analysis.
|
|
Prerequisites
|
unspecified
|
|
Assessment methods and criteria
|
unspecified
Credit: attendance and approved protocols from the practicals
|
|
Recommended literature
|
-
Detailed protocols for practicals.
|