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Lecturer(s)
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Vopátková Věra, Mgr.
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Lencová Radka, Mgr.
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Kovaříková Anna, Mgr.
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Langhansová Helena, RNDr. Ph.D.
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Chmelař Jindřich, doc. RNDr. Ph.D.
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Kopecký Jan, prof. RNDr. CSc.
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Frejlachová Andrea, Mgr.
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Kamiš Jan, RNDr.
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Kotál Jan, Mgr. Ph.D.
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Course content
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Content of lectures 1. Innate immunity - overview of basic functions: role of the immune system, basic concepts, innate and acquired immunity, regulation of immunity. 2. Experimental systems in immunology. Cells and organs of the immune system: lymphocytes, mononuclear phagocytes, granulocytic cells, primary and secondary lymphoid organs, recirculation of lymphoid cells. 3. Antigens: chemical and biological properties, adjuvants, haptens, viral and bacterial antigens, mitogens. Structure and function of immunoglobulins: antigenic determinants on immunoglobulins, Properties and functions of immunoglobulin classes. 4. Antigen-antibody interactions: affinity, avidity, cross-reactivity. Immunochemical reactions. Monoclonal antibodies. 5. Organisation and expression of immunoglobulin genes: generation of antibody diversity. 6. Major histocompatibility complex: structure and function of MHC molecules. T-cell receptor: generation of TCR diversity, MHC restriction, development of T-cells. 7. Cytokines: structure and function of cytokines and their receptors. Development of the humoral immune response: primary and secondary response, antigen presentation, role of cytokines. 8. Cell-mediated immunity: CTL-mediated cytotoxicity, antibody-dependent cell-mediated cytotoxicity, delayed-type hypersensitivity. Regulation of immunity and immunological tolerance: cytokine-mediated and idiotypic, immunosuppression. Immunologic tolerance. 9. The complement system: classical and alternative pathways of complement activation, regulation. Hypersensitive reactions: immediate and delayed hypersensitivity, anaphylaxis, immune complex-mediated hypersensitivity. 10. Vaccines: inactivated, attenuated, subunit, recombinant. Immune response to infectious diseases: viral and bacterial infections, parasitic diseases. 11. Transplantation immunology: transplantation antigens, mechanisms of graft rejection, immunosuppression. Immune response to tumors: malignant transformation of cells, tumor antigens, cancer immunotherapy. Content of practicals 1. Detection of nitric oxide 2. Indirect immunofluorescence 3. Induction and detection of cytokines 4. Hybridoma technology - production of monoclonal antibodies 5. Titration of interferon 6. Enzyme-linked immunosorbent assay (ELISA) 7. TNF bioassay 8. Immunomagnetic separation 9. Flow cytometry 10.Western blotting
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Laboratory
- Class attendance
- 50 hours per semester
- Preparation for exam
- 100 hours per semester
- Preparation for classes
- 10 hours per semester
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Learning outcomes
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Basic course in immunology primarily for undergraduate students. The aim is to introduce students to the structure and function of the immune system, innate and acquired immunity and its regulation. The course is a prerequisite for other follow-up courses such as Clinical Immunology (KME/721), Immunology of Parasitic Infections (KME/501) or Molecular Immunology (KME/087) and assumes prior knowledge of biochemistry and cell biology. The aim of practical course is to master selected methods involving mechanisms of innate and adaptive, humoral and cellular immunity. Special emphasis is put on immunity against infections.
Students will get a basic idea of the structure and function of the immune system.
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Prerequisites
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Biochemistry (UCH/036), Essentials of medical biology (KME/085), basic laboratory skill
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Assessment methods and criteria
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Oral examination
To pass the course, student has to answer at least 70 % questions during the exam and attend at least 80 % of practical classes. Student draws 3 questions and must answer all three at least to grade 3.
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Recommended literature
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Coico R., Sunshine G.: Immunology a Short Course (6th ed.), Wiley-Blackwell 2009, 391 pp..
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J. Krejsek, O. Kopecký: Klinická imunologie, Nucleus HK 2004, 941 str..
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J. Kuby: Immunology (3rd ed.), W.H. Freeman and Company 1997, 664 pp..
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Jiřina Bartůňková Radek Špíšek Tomáš Brdička Václav Hořejší. Základy imunologie, 6. vydání. TRITON, 2017. ISBN 978-80-7553-250-3.
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