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Lecturer(s)
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Course content
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1) Basic characteristics of bionalytical methods and their role in clinical analysis, 2) Physico-chemical properties of metabolites and macromolecules conditioned by intramolecular interactions, 3) Intermolecular interactions in metabolites and macromolecules, 4) Properties of waves and electromagnetic radiation used in bioanalysis, 5) Electroanalytical methods, 6) Sample preparation for analysis, principles of extraction methods, 7) Principle of substance separation, chromatography, 8) Liquid and gas chromatography, 9) Principle of substance separation, electromigration methods, electrophoresis, 10) Immunochemical methods, 11) Mass spectrometry (MS), 12) Interpretation of mass spectra and nuclear magnetic resonance (NMT), 13) Evaluation of the results obtained by analytical measurements. Exercises are complemented by lectures with practical examples of bioanalytical methods and illustrative practical examples.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Practical training
- Preparation for classes
- 10 hours per semester
- Preparation for exam
- 30 hours per semester
- Semestral paper
- 10 hours per semester
- Class attendance
- 39 hours per semester
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Learning outcomes
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The course explains main analytical methods for analysis of substances (analytes) in clinical material so that the student will understand principles of the main instrumental methods used in current bioanalysis. During the course the student will study the physico-chemical properties of a suitable, selected analyte according to the assignment, collects suitable, available information about it, draws the chemical structure in a suitable, available drawing program, describes by him/her proposed method, its preanalytical, analytical and postanalytical phases and relevant references.
Students will gain an overview of basic bioanalytical methods and their application in clinical analysis.
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Prerequisites
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Knowledge from the basic course of physical chemistry and basic introductory practical chemical exercises.
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Assessment methods and criteria
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Combined exam
Submit a seminar paper focused on the analysis of a selected analyte (required for credit), successfully complete a written test (minimum score of 50%) and pass an oral exam (answer 2 out of 3 questions). Attendance at the practicum (maximum 1 absence)
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Recommended literature
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Harris D. C. Quantitative Chemical Analysis. W. H. Freeman, 2015. ISBN 978-1464135385.
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Harris, Daniel C. Exploring chemical analysis. 5th ed. New York : W.H. Freeman and Company, 2013. ISBN 978-1-4292-9576-5.
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Káš, Jan; Kodíček, Milan,; Valentová, Olga. Laboratorní techniky biochemie. 1. vyd. Praha : Vydavatelství VŠCHT, 2005. ISBN 80-7080-586-2.
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KRÁLOVÁ B., RAUCH P. Bioanalytické metody.. VŠCHT, Praha, 2001. ISBN 80-7080-234-0.
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Pawliszyn, J.. Handbook of sample preparation. Hoboken, N. J.: John Wiley, 2010, 491 s.. ISBN 978-0-470-09934-6.
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