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Lecturer(s)
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Franta Zdeněk, RNDr. Ph.D.
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Fessl Tomáš, Mgr. Ph.D.
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Dyčka Filip, Mgr. Ph.D.
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Course content
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Content of lectures: 1. Introduction to biochemical and molecular-biological studies (equipment and safety in the laboratory, GLP, experiment design, nucleic acids: structure and function, genes and genome, isolation and work with NA, PCR, sequencing, basics of bioinformatics) 2. Recombinant DNA in biology (cloning of genes and cloning vectors, gene analysis and expression, expression of foreign genes and preparation of recombinant proteins, types of expression systems basics of cell cultures) 3. Proteins (protein function and structure, enzymes: active sites and catalytic mechanism, control of enzymatic activity, utilization of enzymes) 4. Electrophoretic methods (principles, media, agarose Electrophoresis (DNA, RNA and capillary electrophoresis) SDS-PAGE, native gels, gradient gels, 2D gels, zymography, Western blotting, immunobiochemistry) 5. protein purification and centrifugation (Principles, Types of Chromatography, tags, types of centrifuges, rotors, types of centrifugation 6. Mass spectroscopy techniques I 7. Mass spectroscopy techniques II 8. protein folding and verification of native state 9. spectroscopy methods in biology 10. Microscopy (introduction and types of microscopes, composition of microscopes, optical microscopy (techniques and limitations, resolution, sample preparation, stereomicroscope, confocal microscopy, fluorescence microscopy). Electron microscopy (types and techniques, preparation of samples, limitations), visualization in living cells and tissues Content of practicals: During the course the knowledge gained during the lectures will be enhanced.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, Laboratory, Practical training
- Preparation for classes
- 50 hours per semester
- Class attendance
- 26 hours per semester
- Preparation for exam
- 25 hours per semester
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Learning outcomes
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The course provides a comprehensive introduction to modern methods of biochemistry and molecular biology, with a focus on up-to-date laboratory techniques. Students will gain practical skills in working with both nucleic acids and proteins, as well as in analytical methods (electrophoresis, chromatography, mass spectrometry) and the basics of microscopy. Emphasis is placed on experimental design, good laboratory practice, and the interpretation of results, enabling students to develop the ability to work independently in a research laboratory.
Student should understanding basic principles on techniques in molecular biology a biochemistry according to the specific lectures in the section "Obsah, opory". In practical part, students acquire skills in purification of recombinant proteins, analyzing the purified proteins by SDS-PAGE, measuring protein concentration and activities, advanced MS and optical spectroscopic techniques and data analysis.
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Prerequisites
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Basic knowlegde in biology, biochemistry and chmistry. A minimal laboratory practice - pippeting, preparation of biological buffers, calculation of solution concentrations.
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Assessment methods and criteria
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Student performance assessment
Active understanding of the material covered and active participation in exercises are required. The course includes block (intensive) practical session; the date will be specified at the beginning of the semester. Attendance at the introductory lecture and the block practical session is mandatory. Students are required to work actively in the laboratory and complete all assigned tasks, including processing and calculating results. Only students who successfully complete the laboratory part of the course and submit the required reports are allowed to take the final test. To receive course credit, the student must complete the block practical session and have their submitted report approved by the instructor.
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Recommended literature
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Bruce Alberts, Dennis Bray, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter: Základy buněčné biologie: úvod do molekulární biologie buňky, původně Essential Cell Biology. Second Edition. New York: Garland Science. ISBN: 0-8153-3480-X (hc); 0-8153-3481-8 (pb), 2004..
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https://www.databazeknih.cz/knihy/zaklady-bunecne-biologie-uvod-do-molekularni-biologie-bunky-45040.
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ŠMARDA, Jan. Metody molekulární biologie. 1. vyd. Brno: Masarykova univerzita, 2005, 188 s. ISBN 80-210-3841-1..
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Wilson, Keith, and Walker, John (eds.): Principles and techniques of biochemistry and molecular biology (6th Ed.) Cambridge University Press, Cambridge, 2005, 802 pp., ISBN 0-521-82889-9,P70.00.
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