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Lecturer(s)
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Klementová Šárka, doc. RNDr. CSc.
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Course content
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Optical methods: Spectrophotometry: observation of colours in VIS spectrum, measurement of absorption spectra in VIS and UV region, calculation of molar absorption coefficient, construction of spectrophotometric calibration curve, determination of concentration of unknown samples. Refractometry. Electrochemical methods: Potentiometry - measurement and evaluation of potentiometric titration curve of diprotic acid. Conductometry - determination of conductometry cell constant, measurement of conductivity of water of different purity. Separation methods: Liquid-liquid extraction. Dialysis. Chromatography (partition chromatography).
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Learning activities and teaching methods
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- Class attendance
- 39 hours per semester
- Preparation for classes
- 39 hours per semester
- Preparation for credit
- 30 hours per semester
- Preparation for exam
- 30 hours per semester
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Learning outcomes
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The aim of the course is to master fundamental instrumental techniques used in chemical laboratories.
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Prerequisites
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The mandatory prerequisite is UCH 006.
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Assessment methods and criteria
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unspecified
Students must complete all practical exercises. Students must submit final reports complying with the given rules
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Recommended literature
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Campbell M. Introduction to spectrophotometry. 2021.
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Gross E.M. Kelly R.S., Cannon D. M. Analytical Electrochemistry: Potentiometry. 2023.
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Klementová Š. Návody k praktickým úlohám UCH 120 (2023, každoročně inovováno). .
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