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Lecturer(s)
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Dyčka Filip, Mgr. Ph.D.
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Franta Zdeněk, RNDr. Ph.D.
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Hořejší Karel, Mgr. Ph.D.
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Course content
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Content of lectures: Week 1: Introduction to omics, Genomics (2h) Week 2: Genomics (2h) Week 3: Transcriptomics (2h) Week 4-6: Proteomics (6h) Week 7-8: Lipidomics (4h) Week 9: Metabolomics (2h) Week 10: Reserve
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Learning activities and teaching methods
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- Class attendance
- 20 hours per semester
- Preparation for classes
- 30 hours per semester
- Preparation for exam
- 50 hours per semester
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Learning outcomes
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The course aims to provide students with a comprehensive overview of omics approaches used in system biology in order to understand and develop our understanding of various (patho)physiological processes and mechanisms occurring in living organisms. The emphasis is also placed on modern analytical techniques and procedures used in clinical and biomedical research to screen and monitor biological systems and to reveal previously unknown molecular players in many diseases, including cancer.
Students know the various techniques used in omics (k1), can explain the principles of various techniques (k2), compare, differentiate and evaluate the differences in various methods (k4, k5), and design a protocol for sample analysis for a specific application (k6).
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Prerequisites
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Prerequisites: KMB/758 (Molecular Biology and Genetics I) KMB/605 (Introduction to Bioinformatics) UCH/757 (Biochemistry 1) UCH/643 (Biochemistry 2)
KMB/758 and KMB/605 and UCH/757 and UCH/643
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Assessment methods and criteria
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unspecified
Requirements on student: The course is successfully completed after obtaining at least 50% of the points in the written exam.
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Recommended literature
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Ekroos, K., ed. . Lipidomics: Technologies and Applications, 1st Edition. Weinheim, Germany: Wiley-VCH Verlag (2012).. ISBN 978-3-527-33098-0.
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Han, X. Lipidomics: Comprehensive Mass Spectrometry of Lipids, 1st Edition. Hoboken, New Jersey: John Wiley & Sons (2016)..
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Holčapek, M. and Ekroos, K., eds. Mass Spectrometry for Lipidomics: Methods and Applications, 1st Edition. Weinheim, Germany: Wiley-VCH Verlag (2023).. ISBN ISBN 978-3-527-33.
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Rehm. . Protein Biochemistry and Proteomics, 1st Edition. Elsevier Science Publishing Co Inc (2006).. ISBN 9780120885459.
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Twyman, R. Principles of Proteomics, 1st Edition. London: Taylor & Francis Ltd (2004). ISBN 9781859962732.
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Wu, C. H, Arighi, C. N., and ross, K. E., eds. . Protein Bioinformatics: From Protein Modification and Networks to Proteomics, 1st Edition. New York: Humana Press (2017)..
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