Course: Plant Biochemistry

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Course title Plant Biochemistry
Course code UCH/782
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 3
Language of instruction English
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Bína David, RNDr. Ph.D.
  • Litvín Radek, RNDr. Ph.D.
Course content
Content of lectures: The lecture will consist of several thematic sections covering different aspects of plant function: A) Introduction Biosphere as a solar-powered system, photosynthesis as global geochemical actor (carbon, oxygen); brief sketch of life evolution and its geological record related to photosynthesis (oxygen, coal, oil, carbonates etc.); the tree of life: bacteria, archaea, eukarya, evolution and characteristics of the plant cell. B) Plant function 1. Solar power-plants: the light-dependent reaction of photosynthesis. Basics of photochemistry, thermodynamics, redox chemistry; role of membranes, electron and proton transfer; principles of light utilization: light harvesting, reaction center, cytochromes, ATP synthesis; respiration 2. Enzymatic reactions of photosynthesis, carbon assimilation, RuBisCO, C3, C4, CAM pathways of carbon fixation. Non-RuBisCO-based carbon assimilation pathways from bacteria (e.g. reversed TCA) 3. Gas exchange and water transport. Basics of gas mechanics and hydraulics. Water movement between the plant, soil and atmosphere 4. Metabolism of macro/micronutrients (nitrogen, phosphate, sulfur etc.), metaloenzymes, acquisition of metals from environment; toxicity. C) Plant structure. Having established the main functions of plant organism such as light utilization, gas and water transport we can proceed to description of how the plant body is built in order to carry out its functions: 5. Structure and synthesis of polymer building blocks of the plant body: cellulose, pectin, lignin, proteins, lipids. Regulation of growth - chemistry of plant hormones. Basics of signal transduction (G-proteins etc.) regulation of gene expression. Plant growth and development, its regulation by environmental cues (e.g. response to light, gravitation). D) 6. Non-plant phototrophs - algae (and also bacteria); global importance for geochemical cycles, symbiotic interactions (e.g. coral reefs), secondary metabolites, algal blooms E) Plants in biotic interactions. 7. Plants-viruses, viroids; plants-bacteria (nitrogen fixation, Agrobacterium), plant-fungi (mycorrhiza, diseases); plant-plant (allelochemicals); plants-animal (defense, attraction, alkaloids, non-photosynthetic pigments etc.) 8. Plants and humans. Agriculture, mechanisms of herbicide action, environmental burden; biotechnology (resistance, metabolism, production of valuable compounds).

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Written action (comprehensive tests, clauses), Individual preparation for exam, Work with multi-media resources (texts, internet, IT technologies)
  • Class attendance - 20 hours per semester
  • Preparation for exam - 20 hours per semester
Learning outcomes
Provide the fundamental understanding of the chemistry of mechanisms of functioning of the plant organism from cellular level to ecological community. Energy generation, regulation of development as well as interaction with both biotic and abiotic factors of environment will be covered.
Students will gain knowledge of plant biochemistry and must pass an exam.
Prerequisites
Introductory-course level knowledge of biochemistry, physical chemistry and cell biology

Assessment methods and criteria
Oral examination, Seminar work

In addition to the final exam, there will be written assignments (~2-3 per semester) consisting of a computation problem set and a written essay on a selected topic from scientific literature.
Recommended literature
  • Blankenship R. E. Molecular mechanisms of Photosynthesis. Blackwell, 2002. ISBN 0-632-04321-0.
  • Heldt, Hans-Walter; Heldt, Fiona. Plant biochemistry. Third edition. Amsterdam ; Boston ; Heidelberg ; London ; New York ; Oxford ; Paris ; San Diego ; San Francisco ; S, 2005. ISBN 0-12-088391-0.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester