1 Introduction: Summary of basic molecular biology principles. 2 RNA world: Overview of coding and non-coding RNA, RNA secondary structures, ribozymes, siRNA, miRNA, lncRNA, mRNA cycle in the cell - capping, splicing, translation regulation and degradation; RNA interference. 3 Protein life cycle in the cell: Protein folding, chaperone, protein trafficking and targeting in the cell, protein turnover, degradation, proteasome, protein posttranslational modifications. 4 Membranes: Composition of membranes, membrane transport, lipid rafts, respiratory chain. 5 Regulation of gene expression: Transcription, transcription factors, repressor, activator, operons, histone modifications, DNA modifications, epigenetic regulation, DNA methyltransferase, CpG islands. 6 Developmental 1: Ontogenesis, development, primary cells, polarization. 7 Developmental 2: From cell to tissue, cell differentiation, types of differentiation, cytoskeleton. 8 Hormones: Endocrine glands and endocrine signalling, protein hormones, steroid hormones, derivatives of the amino acid tyrosine, controls of hormone secretion, negative/positive feedback, cyclic changes, mechanism of action of hormones, functional disorders of endocrine glands. 9 Cell signalling: Types of signalling and types of receptors for sensing, protein-coupled receptors, receptor tyrosine kinases, integrin signalling, signal transduction, cell signalling and apoptosis, signalling pathways and signal response. 10 Cancer: Cancer cell features, tumorogenesis, metastasis, oncogene, tumour suppressor genes, examples from human medicine, viruses and cancer, hereditary cancer diseases, Cancer treatment. 11 Developmental Biochemistry - Examples of development and adaptation of biochemical metabolic pathways across groups of organisms. 12 Advanced molecular biology methods: RNAi, CRISPR/Cas9, gene therapy.
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Individual preparation for exam, Work with multi-media resources (texts, internet, IT technologies)
- Class attendance
- 39 hours per semester
- Preparation for classes
- 35 hours per semester
- Preparation for exam
- 50 hours per semester
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Alberts B. Molecular Biology of the Cell.. W. W. Norton & Company, 2014.
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D. L. Nelson, M. M. Cox. Lehninger Principles of Biochemistry, 6. edition. New York, 2013. ISBN 1-4292-3414-8.
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Darnell, James E.; Lodish, Harvey F.,; Baltimore, David. Molecular cell biology. 2nd ed. New York: Freeman, 1990. ISBN 0-7167-2078-7.
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Gilbert, S. F. Developmental Biology. Sinauer Associates Inc., 2006. ISBN 0-87893-250-X.
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Krebs, Jocelyn E.; Goldstein, Elliott S.; Kilpatrick, Stephen T.; Lewin, Benjamin. Lewin's genes XII. Twelfth edition. Burlington: Jones & Bartlett Learning, 2018. ISBN 978-1-284-10449-3.
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