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Lecturer(s)
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Polívka Tomáš, prof. RNDr. Ph.D.
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Litvín Radek, doc. RNDr. Ph.D.
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Bína David, RNDr. Ph.D.
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Course content
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The course focuses on introducing optical spectroscopy methods, including the theoretical foundation of individual methods and their application, particularly for studying biological and biochemical systems. Lectures are supplemented with practical exercises demonstrating the use of individual methods. Content: 1. Radiation, light-matter interaction, absorption, emission, scattering 2. Radiation sources, detection methods, principles, applications 3. Methods based on light scattering by macromolecules, DLS 4. Absorption spectroscopy 5. Linear and circular dichroism 6. Fluorescence 1, fundamentals of the method: excitation emission spectra, fluorophores, FRET 7. Fluorescence 2, time-resolved fluorescence, fluorescence correlation spectroscopy 8. Vibrational methods, introduction 9. Infrared spectroscopy, FTIR 10. Raman spectroscopy methods, resonance Raman spectroscopy 11. Ultrafast spectroscopy: transient absorption, photon echo, two-dimensional spectroscopy (optional) 12. Optical microscopy, confocal microscopy, localization, high resolution
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Demonstration, Laboratory
- Class attendance
- 52 hours per semester
- Preparation for exam
- 50 hours per semester
- Preparation for classes
- 50 hours per semester
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Learning outcomes
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This course introduces methods of optical spectroscopy used primarily for the study of organic molecules and biological systems. Lecturer will demonstrate the theoretical fundamentals and the methods were applied at limit frontier of theoretical availability. Instrumentation is involved in practicals.
Students intensify their knowledge of optics, optical instrumentation techniques and methods used primarily for biological and biochemical materials.
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Prerequisites
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Basic knowledge of Physics III topics (wave and particle nature of light, interference and diffraction, wave theory, refraction index and polarization)
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Assessment methods and criteria
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Oral examination, Development of laboratory protocols
Part of the course Optical Spectroscopy is a regular, one-week, practice. All protocols on practices are required. Oral exam.
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Recommended literature
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Gilbert, A., J. Baggott. Essentials of Molecular Photochemistry, 1991..
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H. van Amerongen et al.: Photosynthetic Excitons, 2000..
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P. W. Atkins: Physical Chemistry..
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P.W. Atkins: Molecular Quantum Mechanics..
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Atkins, P. W.; De Paula, Julio; Bartovská, Lidmila; Cibulka, Ivan,; Dohnal, Vladimír,; Chuchvalec, Pavel,; Kania, Patrik,; Kolafa, Jiří,; Kratochvíl, Bohumil,; Malijevský, Alexandr; Matějka, Pavel,; Řehák, Karel,; Slavíček, Petr,; Tkadlecová, Marcela. Fyzikální chemie. Vydání první. Praha: Vysoká škola chemicko-technologická v Praze, 2013. ISBN 978-80-7080-830-6.
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Lakowicz, Joseph R. Principles of fluorescence spectroscopy. 2nd ed. New York : Kluwer: Kluwer, 1999. ISBN 0-306-46093-9.
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Prosser, Václav. Experimentální metody biofyziky. Vydání 1. Praha : Academia: Academia, 1989. ISBN 80-200-0059-3.
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