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Lecturer(s)
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Linhart Otomar, prof. Ing. DrSc.
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Course content
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Study supporting materials at e-learning portal of USB (LMS Moodle): https://elearning.jcu.cz/course/view.php?id=2438
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Learning activities and teaching methods
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Individual preparation for exam, Individual tutoring
- Preparation for classes
- 12 hours per semester
- Class attendance
- 4 hours per semester
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Learning outcomes
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The aim of the study is to make students familiar with reproductions of some of bony and cartilaginous freshwater fish with an emphasis on reproductive physiology, endocrinology, sexuality, gametogenesis, genome manipulation and reproduction of fish in breeding practice. The main topics will be: 1) Spermatogenesis and oogenesis, meiosis and mitosis, induction of maturation, ovulation and spermiation. 2) Controlled spermiation including ovulation and endocrine regulation of the environment. 3) Sexuality in fish (hermaphroditism, unisexuality and bisexuality), sex ratio, changes of sex in natural and breeding conditions and hybridization. 4) Reproduction of fish in breeding practice, the influence of rearing environment, inbred depression, hormones, pheromones and reproductive behavior in fish. 5) Methods of controlled reproduction in freshwater fish species with exogenous hormone treatment and environmental manipulation. 6) Fish gametes, their physiology and preservation of short and long term gametes, including freezing of embryos and stem cells. 7) Activation, fertilization of gametes and incubation of eggs. 8) Genome manipulation - principles of uniparent inheritance and cloning of parental genomes and polyploid and level of gametes manipulation during sowing, activation, fertilization.
After completing the course, students will master the theory and practical reproduction of fish.
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Prerequisites
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The subject has no prerequisites.
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Assessment methods and criteria
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Oral examination
Oral exam; Other requirements: seminar work on the topic of reproduction of selected fish species
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Recommended literature
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Billard, R., et al., 1995. Biology of sperm and artificial reproduction in carp. Aquaculture, 129: 95-112..
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Black, K.D. and Pickering, A.D., (ed)., 1998. Biology of Farmed Fish, CRC Press, Sheffield, 415 s..
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Day, J.G. and McLellan, M.R., 1995. Cryopreservation and freeze drying protocols. Methods in Molecular Biology, 32, Humana Press, Totowa, New Jersey, 254 s..
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Dettlaff T.A. et al., 1993. Sturgeon Fishes: Developmental Biology and Aquculture. Springer-Verlag, London, 300 s..
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Gagnon, C. (ed.)., 1999. The Male Gamete. Cache River Press, Toronto, 516 s..
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Linhart, O. et al., 1995. Morphology composition and fertilization of carp eggs. Aquaculture, 129: 75-93.
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Mellinger J., 2002. Sexualite et Reproduction des poissons. CNRS Editions, Paris, 349 s..
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