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Lecturer(s)
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Křížek Martin, prof. Ing. CSc.
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Beran Pavel, Ing. Ph.D.
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Course content
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I. Gas and liquid chromatography in tandem with mass spectrometry. Supercritical fluid chromatography and extraction. Lateral flow immunochromatographic assay. Capillary electrophoresis of small molecules and proteins. Capillary electrophoresis in conjunction with mass spectrometry. II. Atomic emission spectrometry. Luminescence analysis. Atomic absorption spectrometry. Molecular absorption photometry. Extraction photometry. Infrared spectrometry. NMR. III. Electromigration methods, electroforetic mobility, principles. Electrophoretic separation of biological macromolecules. Isoelectric focusing. 1-D and 2-D electrophoresis. Detection of enzymatic activity on the gel, detection of nucleic acids. AGE, SGE, PAGE, capillary electrophoresis, chip electrophoresis for proteins and nucleic acids. Optical methods, SPR. Immunochemical methods, antibodies, immunoprecipitation and non-precipitation methods. IV. Automatic analysers and their use in analytical chemistry. Statistical processing of data in analytical chemistry.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), E-learning, Work with multi-media resources (texts, internet, IT technologies), Individual tutoring
- Preparation for classes
- 30 hours per semester
- Preparation for exam
- 150 hours per semester
- Class attendance
- 28 hours per semester
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Learning outcomes
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The objective is to provide an overview of selected modern analytical methods used in natural sciences. These methods go beyond the basic analytical chemistry courses and represent a special addition to the other subjects. Special Analytical Methods contribute to the overall professional profiling of the doctoral student.
Students get an overview of selected modern analytical methods related to their professional profiling.
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Prerequisites
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Students should have successfully passed their basic chemistry examinations as part of their master's degree.
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Assessment methods and criteria
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Oral examination
Commanding of the basics of selected parts of analytical chemistry with respect to the necessary understanding of inorganic, organic and physical chemistry.
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Recommended literature
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Harris D. C. Quantitative Chemical Analysis. W. H. Freeman, 2015. ISBN 978-1464135385.
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Křížek M., Šíma J. Analytická chemie. České Budějovice, 2015.
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Kurien B. T., Scofield R. H. Electrophoretic Separation of Proteins: Methods and Protocols. Humana Press, 2019. ISBN 978-1493987924.
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Makovets S. DNA Electrophoresis: Methods and Protocols. Humana Press, 2013. ISBN 978-1627035644.
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Opekar F., Jelínek I., Rychlovský P., Plzák Z. Základní analytická chemie. Praha, Karolinum, 2002.
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Skoog D. A. et al. Fundamentals of Analytical Chemistry. Brooks/Cole, 2013. ISBN 978-0495558286.
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