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Lecturer(s)
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Čítek Jindřich, prof. Ing. CSc.
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Course content
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Introduction to the discipline, historical survey in the embryotransfer. Artificial insemination and its role in livestock breeding. Technological procedure ET in embryos in vivo after superovulation. Limiting factors for embryotransfer method. Physiological sexual cycle. Rut synchronization. Superovulation and repetitionary superovulation. Embryo preservation by freezing. Embryos produced in vitro. Used micromanipulation with embryos. Embryotransfer. Biotechnology of future. Biotechnology usage in animal breeding. Hazard and ethic aspects of biotechnology development. Differences and use of ET findings in cattle, sheep, goats and pigs.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Class attendance
- 56 hours per semester
- Preparation for exam
- 70 hours per semester
- Preparation for credit
- 35 hours per semester
- Semestral paper
- 20 hours per semester
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Learning outcomes
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The aim of the subject is to introduce with contemporary cognizance status about animal biotechnology and perspective developments.
Artificial insemination, synchronisation of estral cycle, embryotransfer, superovulation. Kryoconservation. Micromanipulation with embryos. Importance for breeding. Specific approaches in species of farm animals.
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Prerequisites
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Genetics I - KGV/GEN1, Genetics II - KGV/GEN2, Biochemistry - KCH/BCHVZ
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Assessment methods and criteria
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Oral examination
Knowledge in genetics, molecular biology, gene engineering.
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Recommended literature
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Arumugam N., Kumaresan V. Animal Biotechnology. Saras Publication, 424 pp..
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Bonduriansky R. The genetic architecture of sexual dimorphism: the potential roles of genomic imprinting and condition dependence. Sex, Size And Gender Roles:Evolutionary Studies of Sexual Size Dimorphism. Oxford University Press, Oxford, 2007. 266pp..
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Greve J. Embryo technology: implications for fertility in cattle. Revue Scientifique et Techique Office International des Epooties 24, 2005,1,405-412..
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Howe Ch. Gene Cloning and Manipulation,Second edition. Cambridge University Press, 2007..
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Linhart O. et al. (2006). Insemination, fertilization and gamete management in tench, Tinca tinca (L.). Aquaculture international 14 (1-2): 61- 73..
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Nicholl D. S. T. (2008). An Introduction to Genetic Engineering (Third Edition), Cambridge University Press, 2008, 347pp..
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Noakes, D., Parkinson, T., England, C. Veterinary Reproduction and Obstetrics. Elsevier Sanders, 2018, 848 s..
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Presicce G. Presicce G.: Reproductive Technologies in Animals. Academic Press; 1st edition, 2020, 306 pp..
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Sheep, Horses, Swine and Other Animals. A Handbook & Laboratory Manual for Students Herd Operators & Persons Involved in Genetic Development, 9th Edition. Prentice Hall, 2003..
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Smith J.A. Artificial Insemination & Embryo Transfer of Dairy & Beef Cattle Including Information Pertaining to Goats,.
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