| Course title | Cloning of Animals |
|---|---|
| Course code | KGZB/KTŽ |
| Organizational form of instruction | no contact |
| Level of course | Doctoral |
| Year of study | not specified |
| Semester | Winter and summer |
| Number of ECTS credits | 0 |
| Language of instruction | Czech |
| Status of course | Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Isolation of immature eggs in the embryonic sac stage and their cultivation to the metaphase II stage, isolation of ovulated eggs in the metaphase II stage, isolation of sperm and their preparation for fertilization, preparation of microinstruments for sperm injection. In vitro embryo production and further embryo manipulation. Oocyte culture, "in vitro" fertilization, nuclear transfer, embryo bisection, cryopreservation. General procedures for the preparation of culture and handling media, procedures for culturing, separating and isolating cells. Common staining procedures by immunofluorescence methods.
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| Learning activities and teaching methods |
Monologic (reading, lecture, briefing)
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| Learning outcomes |
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The aim of the course is to acquaint doctoral students with the procedures listed below, not only theoretically, but also to enable them to partially master some basic techniques.
The student can independently navigate in the given issue and is able to use the acquired knowledge in their scientific activities or other practice. |
| Prerequisites |
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The student is familiar with the issues within the scope of university studies and is willing to study independently.
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| Assessment methods and criteria |
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Oral examination
General mastery of mammalian embryo production and cultivation procedures. Mastering the basic procedures of micromanipulations |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Agriculture and Technology | Study plan (Version): Agricultural Biotechnology (1) | Category: Agriculture and forestry | - | Recommended year of study:-, Recommended semester: - |
| Faculty: Faculty of Agriculture and Technology | Study plan (Version): Agricultural Biotechnology (1) | Category: Agriculture and forestry | - | Recommended year of study:-, Recommended semester: - |