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Lecturer(s)
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Hypša Václav, prof. RNDr. CSc.
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Čurn Vladislav, prof. Ing. Ph.D.
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Course content
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Thematic blocks of lectures: Specifics of molecular biological data, their advantages and disadvantages. Acquisition of molecular biological data for the purposes of systematics (sequencing, RFLP, RAPD, AFLP, allozymes, microsatellites, SSCP, reassociation analysis, immunological methods, protein fingerprinting). Processing of acquired data - phenetic and cladistic approaches, distance and character data, methods of distance calculation based on different types of character data, construction of dendrograms, UPGMA, NJ, ML, ME, comparison of two dendrograms, reliability testing of individual branches of dendrograms (jackknifing, OUT-jakknifing , bootstrapping). Biological interpretation of results. Software for molecular phylogenetics.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Semestral paper
- 90 hours per semester
- Preparation for classes
- 50 hours per semester
- Preparation for exam
- 50 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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The aim of the course is mastering of the methods and techniques of molecular phylogenetics, with the aim to use molecular biological data in the systematics and related biological fields.
The student can independently navigate in the given issue and is able to use the acquired knowledge in their scientific activities or other practice.
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Prerequisites
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The student is familiar with the issues within the scope of university studies and is willing to study independently.
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Assessment methods and criteria
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Oral examination
Standard knowledge of genetics and statistics
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Recommended literature
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Avise, J. C. Molecular Markers, Natural History and Evolution. New York, Chapman & Hall 1993, 684 s..
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Graur, D., Li, W. H. Fundamentals of Molecular Evolution. Massachusetts, Sunderland Massachusetts 1999, 481 s..
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Hillis, D. M., Moritz, C., Mable, B. K. Molecular Systematics. Sinauer Assocites, Inc., Massachusetts, Sunderland Massachusetts 1996, 655 s..
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Li, W. H. Molecular Evolution. Sinauer Assocites, Inc., Massachusetts, Sunderland Massachusetts 1997, 487 s..
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