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Lecturer(s)
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Čurn Vladislav, prof. Ing. Ph.D.
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Beran Pavel, Ing. Ph.D.
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Jozová Eva, Ing. Ph.D.
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Course content
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Definition of plant biotechnology. History of plant cultures in vitro. Plant cell, organ and tissue cultures in vitro, methods of transformation of genetic information. Principles of aseptic manipulation with plant material, facilities in biotechnological laboratory. Nutrition requirements of in vitro cultures. Hormonal composition of cultivation media. Physical conditions of cultivation, solid and liquid media. Cell and suspension cultures. Regulation of morphogenesis in cultures in vitro, regeneration via organogenesis, somatic embryogenesis and androgenesis. Genetic and phenotypic variation of in vitro cultures, epigenetic changes, in vitro selection, somaclonal variation. Haploid cultures, androgenesis, gynogenesis, cultures of anthers and microspores. Genetic manipulation with plant cell. Cultures of plant protoplasts. Biotechnological approaches in plant breeding. Methods conserving stability of genotype, biotechnological methods for increasing genetic variability.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Preparation for credit
- 10 hours per semester
- Preparation for exam
- 20 hours per semester
- Semestral paper
- 20 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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Introduction to plant biotechnology, principles of in vitro cultivation, plant cell, tissue and organ cultures. Utilisation of plant biotechnology in plant breeding.
Students are able to orientate in the field of plant in vitro techniques
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Prerequisites
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Cultivation of plant cell and tissue
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Assessment methods and criteria
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Oral examination
Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
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Recommended literature
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Čurn V. Rostlinné biotechnologie, I. Fyziologie a genetika rostlinných explantátových kultur. Učební texty. 2015.
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David P Clark. Nanette Jean Pazdernik. Biotechnology: applying the genetic revolution. Academic Press/Elsevier, 2009..
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Novák F. J. Explantátové kultury a jejich využití ve šlechtění rostlin. Academia, Praha, 1990.. Academia, Praha, 1990.
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Trigiano, R.N., Gray, D.J. Plant development and biotechnology. CRC Press Boca Raton, 358s. 2007..
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