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Lecturer(s)
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Čurn Vladislav, prof. Ing. Ph.D.
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Course content
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Topics of lectures: I. Organization and structure of genome. Mendelian and molecular genetics. Identification of genes/traits donors. Genome mapping, physical and cytogenetic maps. Identification and isolation of genes. Hybridization techniques, RFLP, FISH, in situ PCR, sequencing, cloning. II. Methods of genome editing, study of gene function. Regulation of gene expression, methods of study. Isolation of mRNA, RT-PCR, qPCR, evaluation of expression. Accumulation of protein, isolation and detection. Bioinformatics. III. Structural and functional genomics. Comparative and population genomics. Metagenomics. Chromosomal genomics. Next generation sequencing. FCM and chromosome sorting. Analysis of transcriptome. IV. Molecular markers. High throughput techniques. Microarrays in SNP genotyping. Perspectives, whole genome sequencing. GWAS and GEM. Associative transcriptomics.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Preparation for classes
- 50 hours per semester
- Preparation for credit
- 50 hours per semester
- Preparation for exam
- 60 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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The aim is to give general knowledge on the trends in molecular genetics, especially in gene and genome analysis of farm animals.
Students will be familiar with the issues of methods of molecular biology and their application in animal genome analysis. Students will be able to define the principles of methods of molecular biology, genomics and transcriptomics. They will also be familiar with the issue of molecular markers and their applications in genomic selection
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Prerequisites
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Standard knowledge of molecular biology and genetics.
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Assessment methods and criteria
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Combined exam
Practical laboratory training, mastering the curriculum defined for the exam.
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Recommended literature
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Alberts B. Molecular Biology of the Cell.. W. W. Norton & Company,, 2014.
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Gregory T. R. The Evolution of the Genome. Academic Press, 2004. ISBN 978-0123014634.
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Lesk A. Introduction to Genomics. Oxford University Press, 2017. ISBN 978-0198754831.
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Lewin, B. Genes VI. Univ. Press, 990 s., 1997.
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On-line studijní texty: http://biocentrum.zf.jcu.cz/ruzne.php. On-line studijní texty: http://biocentrum.zf.jcu.cz/ruzne.php.
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Reece R.J. Analysis of genes and genomes. Wiley Publ., 2004.
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Samuelsson, Tore. Genomics and bioinformatics : an introduction to programming tools for life scientists. 1st pub. Cambridge : Cambridge University Press, 2012. ISBN 978-1-107-00856-4.
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