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Lecturer(s)
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Hoštičková Irena, Ing. Ph.D.
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Čítek Jindřich, prof. Ing. CSc.
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Jozová Eva, Ing. Ph.D.
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Stehlíková Dagmar, Ing. Ph.D.
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Course content
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1. Chemical components of nucleic acids, sugar components, bases, nucleosides, nucleotides. 2-3. Deoxyribonucleic acids, chemical structure, supercoiled formation. 4-5. Ribonucleic acids. RNA biosynthesis, transcription unit, promoters and their structure, transcription origin, terminators, mono- and polycistronic units. 6. Transcriptional apparatus of posttranscriptional editing. 7-8. Protein biosynthesis, genetic code, initiation, termination codons, modified nucleosides in the anticodon loop, suppressors, ORF, the universality of the code. 9-10. Translational apparatus. DNA biosynthesis, replication units, replication apparatus, Okazaki fragments, DNA polymerases. 11-12. Regulatory mechanisms, regulatory genes and their products, positive and negative regulation, activation and repression. 13-14. Regulation at the posttranscriptional, translational, posttranslational level, methods of regulation
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Preparation for credit
- 35 hours per semester
- Preparation for exam
- 80 hours per semester
- Semestral paper
- 30 hours per semester
- Class attendance
- 65 hours per semester
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Learning outcomes
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The aim of the course is to gain knowledge about biopolymers, their structure and function - gene expression and their regulation. Modification of genetic information.
Basis of molecular biology for subsequent study of gene engineering, biotechnologies etc.
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Prerequisites
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genetics - GEN or genetics - GENZO
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Assessment methods and criteria
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Combined exam
Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
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Recommended literature
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Darnell, J. a kol. Molecular Cell Biology. Sci. Am. Books, 930 s., 1990.
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Lewin, B. Genes VI. Univ. Press, 990 s., 1997.
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Rosypal, S. Úvod do molekulární biologie I-III. Brno, náklad vlastní, 620 s., 1997.
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