Lectures: 1. Clinical biochemistry and its position among laboratory disciplines. 2. Properties of laboratory methods in terms of analysis and clinical approach. Sensitivity (sensitivity), specificity, efficiency test, the relationship between sensitivity and specificity, other indicators for assessing laboratory test. 3. Metabolism of water, sodium, potassium and chloride, balance monitoring. Osmolality. 4. Analytical systems used to investigate the internal environment of a clinical laboratory and patients' bedside 5. Acid-base balance and its disorders, blood gases. Metabolism of oxygen. 6. Metabolism of calcium, magnesium and phosphorus. 7. Basic kidney functions and their disorders, glomerular filtration rate, tubular function, laboratory calculations. Non-protein nitrogen compounds - urea, creatinine, uric acid, ammonia, amino acids. Diuresis - distinguish changes, acute and chronic renal failure, measuring the effectiveness of hemodialysis, haemofiltration and peritoneal dialysis, renal transplantation. 8. Biochemical analyzers, analytical systems for routine biochemistry. 9. Plasma proteins. The importance of plasma proteins, individual blood plasma proteins, acute phase reaction. Protein instrumental analysis. 10. Cardiovascular system. Risk factors for the development of atherosclerosis. 11. Laboratory diagnosis of ischemic heart disease - the use of laboratory procedures. 12. Laboratory tests in diabetes mellitus (DM). Instrumental diagnostics of DM, determination of glucose and other suitable analytes. Determination of autoantibodies, early diagnosis of complications, DM and pregnancy, other causes of hypo- and hyperglycemia. 13. Immuno-histochemical analysis systems. 14. Laboratory tests in diseases of the liver. Laboratory signs of hepatocyte damage, malfunction of hepatocytes, toxic liver damage, laboratory signs of cholestasis, laboratory evidence of liver fibrosis, a typical laboratory findings in some liver diseases. Differential diagnosis of hyperbilirubinemia. 15. Laboratory signs of a malignant neoplasm. 16. Clinical and biochemical examination of the digestive tract. 17. Laboratory tests in pregnancy. Adaptive response of the organism to pregnancy, changes in physiological range of laboratory tests in pregnancy. The systems used for diagnostics. Special laboratory investigations in childhood and old age. 18. Examination of cerebrospinal fluid, used laboratory procedures 19. Hormones. General knowledge of the metabolism and determination of hormones, hormones of the hypothalamus, pituitary and pineal gland, thyroid hormones, adrenal hormones, gonads and sex hormones, tissue hormones. Special analyzers. 20. Trace elements. Iron (Fe), copper (Cu), zinc (Zn), selenium (Se), and other trace elements. Vitamins. 21. Laboratory diagnosis in intensive care. Reference values of laboratory tests. Definitions, basic concepts, methods to determine the reference range, the method of comparing the measured values with the reference values, practical notes to determine reference ranges and evaluation of results 22. Quality control laboratory tests.
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DASTYCH, BREINEK a kol.:. Klinická biochemie bakalářský obor zdravotní laborant, MU lékařská fakulta, Brno 2008, ISBN-13: 978-80-210-4572-9.
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RACEK et al.:. Klinická Biochemie, Galén, Praha 2006, ISBN 80-7262-324-9.
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ŠTERN a kol.:. Obecná a klinická biochemie pro bakalářské obory studia, Karolinum 2005, ISBN 978-80-246-1510-3.
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ZIMA, KAZDA, PRŮŠA et al. Laboratorní diagnostika, Galén, Praha 2007, ISBN 978-80-7262-372-3.
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