Lectures (12 two-hour blocks): 1. Introduction (definitions, history, Koch's postulates, the course of infectious disease) Components of pathogenicity, phases of microbiological examination; interpretation and communication of results 2. Immune system and immunology in relation to medical microbiology 1 - components of IS, normal flora (microbiome) Immune system and immunology in relation to medical microbiology 2 - defence against bacteria, viruses, parasites and fungal pathogens; use in laboratory diagnostics of infectious diseases (immunological methods) 3. Immunization - active and passive 4. Antimicrobial agents with an emphasis on ATB; antimicrobial susceptibility testing, ATB resistance 5. General bacteriology (prokaryote x eukaryote, bacterial cell structure, metabolism, growth cycle, etc.) 6. Examination in bacteriology - cultivation and identification in general (methods based on immunological, biochemical, molecular, mass spectrography) 7. Special bacteriology - G + and G- cocci Special bacteriology - G-rods (enterobacteriaceae and other G-rods except anaerobic ones), G + rods, anaerobes (G- and G +) 8. Special bacteriology - spirochaets, mycoplasmas, chlamydia, rickettsia, bartonelles, mycobacteria 9. General virology + examination methods in virology 10. Special virology - 1. (enteroviruses, respiratory RNA viruses, influenza virus, MMR) Special virology - 2. (RNA viruses causing intestinal infections; rabies virus; HIV Special virology - 3. (arboviruses; viral hepatitis) Special virology - 4. (other DNA viruses); Prions 11. Basics of mycology, selected agents of fungal diseases, mycological examinations 1 Basics of mycology, selected pathogens of fungal diseases, mycological examinations 2 12. Basics of parasitology, selected pathogens of parasitic diseases, parasitological examinations 1 Basics of parasitology, selected pathogens of parasitic diseases, parasitological examinations 2 Training (10 four-hour blocks): 1. work safety in the microbiological laboratory, sterilization and disinfection, preanalytical phase of microbiological examination 2. microscopy, preparations for light microscopy, the most common staining used in microbiological examination 3. collection of material, cultivation and cultivation soil 4. description of grown cultures, ATB susceptibility testing 5. G + and G- cocci 6. G + and G- cocks - continued 7. G-rods - enterobacteria + other G-rods (except anaerobes) 8. G-rods - enterobacteria + other G-rods (except anaerobes) - continued 9. Mycology - yeast Mycology - fungi 10. Parasitology
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Students will get acquainted with the theoretical foundations of microbiology with an emphasis on human pathology: - the morphology of microorganisms, physiology of bacterial cultures and the complexity of bacterial metabolism - the basics of anti-infective immunity will be explained - antibiotics in terms of microbiology and the basics of prevention and prophylaxis of infectious diseases - individual fields of medical microbiology - bacteriology, virology, parasitology and mycology; - parts of microbiological examination will be explained, i.e. non-analytical, analytical and interpretive - in the practical part they will focus on the analytical part of the examination (practical laboratory training)
Upon completion, students will: - be able to describe the morphology of microorganisms, the physiology of bacterial cultures and the complexity of bacterial metabolism - recognize the basics of anti-infective immunity - define what antibiotics are in terms of microbiology and the basics of prevention and prophylaxis of infectious diseases - orientate themsleves in individual fields of medical microbiology - gain a practical overview of examination laboratory microbiological methods - link infectious diseases and their possible causes within human organ systems - determine clinically and microbiologically valid samples, indications for their collection and correct collection, processing in the laboratory - expected results
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BENEŠ, J. et al. Infekční choroby.. Praha: Galén, 2009. ISBN 978-80-7262-644-1.
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VOTAVA, M. Lékařská mikrobiologie obecná. NEPTUN Brno, 2001. ISBN 80-902896-2-2.
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Votava, Miroslav. Lékařská mikrobiologie speciální. Brno : Neptun, 2003. ISBN 80-902896-6-5.
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