Course title | Methods in Microbial Molecular Ecology |
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Course code | KBE/261 |
Organizational form of instruction | Lecture + Lesson |
Level of course | Doctoral |
Year of study | not specified |
Frequency of the course | In each academic year, in the summer semester. |
Semester | Summer |
Number of ECTS credits | 4 |
Language of instruction | English |
Status of course | Compulsory-optional |
Form of instruction | Face-to-face |
Work placements | This is not an internship |
Recommended optional programme components | None |
Lecturer(s) |
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Course content |
Content of lectures: 1. Specifity of DNA/RNA isolation from soil and water ecosystems 2. PCR amplification of spec. regions DNA of Bacteria and Archaea 3. Online databases (RDP, GenBank, SILVA) 4. DGGE/TGGE - fingerprinting of microbial communities 5. qPCR -quantitative PCR (absolute quantification of selected genes) 6. EEA - extracellular enzyme activities - connection with decomposition of organic matter Content of practicals 1. DNA/RNA isolation from soil and water ecosystems 2. PCR amplification of spec. regions DNA of Bacteria and Archaea 3. DGGE - fingerprinting of microbial communities 4. qPCR -quantitative PCR (absolute quantification of selected genes) 5. EEA - measuring of extracellular enzyme using MUP-substratets
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Learning activities and teaching methods |
Monologic (reading, lecture, briefing), Practical training
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Learning outcomes |
Objective of the curse is to introduce advanced molecular methods which help to study the huge quantity and diversity of microbial word in different environments
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Prerequisites |
knowledge of basic genetics and molecular biology
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Assessment methods and criteria |
Test, Development of laboratory protocols
basic genetic and molecular biology courses biology of microorganisms |
Recommended literature |
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Study plans that include the course |
Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
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Faculty: Faculty of Science | Study plan (Version): Hydrobiology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Ecosystem Biology (1) | Category: Ecology and environmental protection | - | Recommended year of study:-, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Ecosystem Biology (1) | Category: Ecology and environmental protection | - | Recommended year of study:-, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Hydrobiology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Ecosystem Biology (1) | Category: Ecology and environmental protection | - | Recommended year of study:-, Recommended semester: Summer |
Faculty: Faculty of Science | Study plan (Version): Hydrobiology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |